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- A real time fluorescent PCR(ATF-PCR) method with TaqMan probe that was designed within rDNA-ITS2 was established to detect Pine Wood Nematode, Bursaphelenchus xylophilus. 以松材线虫rDNA -ITS2为靶区 ,建立松材线虫的TaqMan探针实时荧光PCR检测方法。
- real time fluorescent quantitation PCR 实时荧光定量PCR
- real time fluorescent quantitative PCR 实时荧光定量PCR
- Methods A model of animal experiment,a kit for sexdeter mining region Y (SRY) and a set of standard sample were developed for drawing standard curve by real time fluorescence quantitative PCR. 方法结合动物实验模型,设计性别决定因子Y(SRY)实时荧光定量聚合酶链反应(PCR)检测血细胞嵌合率的方法,建立质控标准品,使用实时荧光定量PCR技术,获得标准曲线。
- Real time fluorescence quantitative PCR 实时荧光定量PCR
- real time fluorescent quantitative RT-PCR 实时荧光定量RT-PCR
- Real time fluorescent polymerase chain reaction 实时荧光定量聚合酶链反应
- real time fluorescent quantitation polymerase chain resction 实时荧光定量聚合酶链反应
- real time fluorescent PCR 实时荧光PCR
- Methods Indirect fluorescent staining, FCM analysis and RT PCR were employed to detect the expression and regulation of PTA1 at the levels of PTA1 protein and PTA1mRNA. 用RT PCR方法检测内皮细胞中PTA1mRNA的表达 ;
- Real time fluorescent RT- PCR 实时荧光RT-PCR
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- In-situ real time fluorescence monitoring 原位实时荧光监测
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- Real - time fluorescence quantitative RT - PCR 实时荧光定量PCR
- MRNA Quantitative determination of human telomerase reverse transcriptase mRNA in laryngeal squamous cell carcinoma with real time fluorescent RT-PCR 实时荧光RT-PCR检测喉鳞状细胞癌组织端粒酶逆转录酶
- Keywords Children;Acute leukemia;MDR;P-gp;Real time fluorescent quantitation polymerase chain reaction;MDR function experiment; 儿童;急性白血病;多药耐药;P-糖蛋白;实时荧光定量聚合酶链反应;多药耐药功能实验;