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- The reverse frequency of donor and receptor for auxotroph and resistance is less than 10~(-9), protoplast transformation is carried out in SMM containing 30% oolyethene glycol (MW 6000) and 20mg/ml DNA at controtledtime and temperature. 转化子有的是原养型,有的在含利福平(10单位/亳升)和链霉素(200单位/亳升)的培养基上能生长,也有的兼而有之。
- PEG-mediated protoplast transformation PEG介导转化
- Keywords Bacillus pumilus;pBX96;Protoplast transformation;Alkaline protease; 短小芽孢杆菌;质粒;原生质体转化;碱性蛋白酶;
- The Construction of A E.colt Strain with K88ac LT-B and O149 Antigen Genes by Using Protoplast Transformation Methods 用原生质体转化技术构建含有大肠杆菌K88ac、LT-B和O_149抗原基因的菌株
- A fresh coat of paint can transform a room. 房间重新粉刷一遍可大为改观。
- Protoplast Culture and Genetic Transformation of Zea mays L. 笋玉米原生质体培养及遗传转化的研究。
- protoplast transformation 原生质体转化
- PROTOPLAST PREPARATION FORM DIASPORANGIUM SP. 轮梗霉原生质体的制备。
- The transformation of that project has been finished. 那个项目的改造已经完成。
- There was a noticeable transformation in his appearance. 他的容貌有了明显的变化。
- Susceptible to transformation by growth and development. 能随成长而变形的对成长和发展的变化敏感的
- Decomposition or transformation of a compound caused by heat. 热解化用,高温分解由于热量而导致的化合物的分解或转化
- Keywords avermectin;protoplast fusion;protoplasts transformation;Vitreoscilla hemoglobin;fermentation;dissolved oxygen; 阿维菌素;原生质体融合;原生质体转化;透明颤菌血红蛋白;发酵;溶氧;
- Application of He-Ne Laser in Heterogeneous Protoplast Fusion[J]. 引用该论文 朱振华;胡欣荣;陈五岭;陈亮.
- To transform a metal into a mineral by oxidation. 使矿化通过氧化使金属转化为一种无机物
- Water can transform a desert into a garden. 水能把一片不毛之地变成一座花园。
- A transformation, as by magic or sorcery. 形变如通过魔术或巫术引起的变形
- Heat can transform water into steam. 热能使水变为蒸汽。
- A little paint will transform this old car. 喷点漆就能让这辆旧车变个模样。
- We must transform passivity into initiative. 我们变被动为主动。