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- The recent developments of protein refolding by SEC were reviewed in this paper. 本文对用SEC复性的进展进行了评述,其内容包括SEC复性的原理及其复性过程中的影响因素,并对其未来发展进行了展望。
- The “collapse-rearrangement” mechanism of the protein refolding assisted polymer is proposed. 折叠助剂与蛋白质复合物的解离则可导致蛋白质结构重排,合适的疏水性可以保证蛋白质以合适的速率重排。
- In the liquid chromatography-based protein refolding procedure, a high yield could be achieved at high protein concentrations. 液固层析辅助蛋白质复性可在高蛋白浓度下操作,适用于大规模生产,同时还能使目标蛋白得到部分纯化。
- The protein refolding assisted by the molecular chaperone systems is promised to improve the protein renaturation efficiency. 蛋白质复性,即如何高效地将无活性的包涵体转变成为有活性的蛋白质,已成为基因工程蛋白产业化的瓶颈。
- Investigations on protein refolding by size-exclusion chromatography (SEC) was carried out by using lysozyme as a model system. 摘要以溶菌酶为模拟体系对体积排阻色谱法进行蛋白质折叠过程实验研究。
- In recent years, chromatography has been gradually used in protein refolding from denatured state to biologically active conformation. 近年来层析技术的一个拓展是用于辅助蛋白质从变性态恢复到活性态,即所谓的复性,取得了令人瞩目的进展。
- It contains the mechanism of protein refolding by SEC, the factors affecting protein refolding, the future of this method, one table and 27 references. 文中包括一个表和27篇文献。
- Recently,with the development of studying in mechanism of protein refolding,some new strategies were reported to solve this problem. 近年来,随着对包涵体体外复性机制的深入研究,从包涵体中复性重组蛋白质已经有了很多的策略和方法。
- The high yield of lysozyme activity obtained at high starting concentration illustrates the workability of this method for high concentration protein refolding. 复性溶菌酶与天然溶菌酶色谱保留体积的差异揭示出折叠过程中无活性蛋白质聚集体的存在及其向复性蛋白质转化的机制;
- The protein refolding process assisted by the SMART polymer as artificial chaperone is abstracted as a conformational transition of protein in a confined space. 首先将人工分子伴侣辅助蛋白质的折叠过程抽象为蛋白质在限制性空间中的构象转换。
- The key to engineering practice of protein refolding in vitro is to inhibit the formation of protein aggregates and misfolded proteins. 蛋白质体外折叠技术要解决的关键问题是避免蛋白质的错误折叠以及形成蛋白质聚集体。
- Studies of Protein Refolding Facilitated by Folding Aids 辅助因子促进蛋白质复性的研究
- Keywords inclusion body;protein refolding;additives; 包涵体;蛋白质复性;添加剂;
- Keywords protein refolding;surfactant;lysozyme;CTAB; 蛋白质复性;表面活性剂;溶菌酶;CTAB;
- You need more protein to build you up. 你需要增加蛋白质以增强体质。
- Recent Developments of Protein Refolding by Size Exclusion Chromatography 蛋白质的排阻色谱复性的新进展
- The protein that is a constituent of hemoglobin. 球蛋白由血红蛋白构成的蛋白
- A protein normally present in milk. 乳蛋白质一种通常存在于乳汁中的蛋白质
- Try to balance your diet by eating more fruit and less protein. 多吃些水果,少摄入些蛋白质,使饮食均衡合理。
- Eggs have a high protein content. 鸡蛋含有很高的蛋白质