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- Objective: 1.Subclone B7.2(IgV+C),IgV and IgC domains and construct prokaryotic expression system of them. 2. 目 的： 1．亚克隆B7.;2分子的胞外(IgV+C);IgV及IgC各区段并构建其原核表达载体。
- Objective To clone the flagellar biosynthesis genes flhA,flhB_2 and fliR of Leptospira interrogans and construct their prokaryotic expression system. 目的克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR,并构建其原核表达载体。
- Objective: To obtain the recombinant augiogenin (Ang) protein by means of prokaryotic expression system and investigate its bioactivity. 目的:利用原核表达系统获得重组血管生成素 (Ang)并研究其生物学活性。
- We reconstructed the gene of human scFv (VL-VH) and prepared scFv(VL-VH) and scFv (VH-VL) antibody fragments by prokaryotic expression system PET22b(+)/BL21(DE3). 对筛选获得的人源抗G5-24 scFv(VL-VH)基因进行了重构，将它们克隆入表达载体pET22b(+)中，转化大肠杆菌BL21(DE3) ，IPTG诱导表达；
- This experiment successfully constructed high performance Gly-Gln polymers prokaryotic expression system, whose expression level achieved 40% of total bacterial protein. 本试验成功构建了Gly-Gln多聚体原核表达系统，该系统能够高效表达Gly-Gln多聚体蛋白，表达的目的蛋白占总蛋白的40%25左右。
- In addition, the metamer lectin expressed by prokaryotic expression system did not agglutinate rabbit red blood cells. 原核表达系统获得的凝集素亚基不能凝集兔血红细胞。
- To further inquire into the functions of Mar-HSP70, we constructed Mar-HSP70-pET-28a (+) prokaryotic expression system, and successfully expressed the recombinant Mar-HSP70 in E. 而对于Mar-HSP70 mRNA水平仅对35℃热刺激才大量增加,并且这种上调主要集中在中枢神经、肝胰腺和肌肉组织中;
- Conclusion A prokaryotic expression system of T.v ap33 gene has been established.The expressed fusion protein AP33 shows satisfactory antigenicity and immunogenicity. 结论 构建了阴道毛滴虫ap33基因原核表达系统,重组融合蛋白AP33具有良好的抗原性和免疫原性。
- The chimeric antigen produced by prokaryotic expressing system will be promisingly used to detect and diagnose AIDS in our country. 本论文利用原核表达体系制备的嵌合抗原有望用于我国艾滋病的检测与诊断；
- prokaryotic expression system of pGEX pGEX原核表达系统
- Using the prokaryotic expression system, the ICL gene of Mycobacterium tuberculosis H37Rv was successfully cloned and expressed, which build the basis for screening new anti-tuberculosis drugs with ICL as the target point. 利用原核表达系统成功克隆表达了结核杆菌H37Rv的ICL,为以ICL为靶点建立新型抗结核药物奠定了基础。
- Eukaryonized prokaryotic expression system 真核化的原核表达系统
- RIP Prokaryotic expression system 核糖体失活蛋白
- The prokaryote expression system is used to express firstly functional B7.2 peptide fragments and demonstrate that the expressed products has similar costimulatory biological activity. 3 . 2．首次用原核系统表达出具有生物活性的共刺激分子B7.;2。
- Construction of prokaryotic expression system of ltB-ureB fusion gene and identification of the recombinant protein immunity and adjuvanticity ltB-ureB融合基因原核表达系统的构建及其产物免疫性和佐剂活性的鉴定
- Keywords human mammaglobin;breast cancer;prokaryotic expression system;protein purification;hybridoma;monoclonal antibody; 人乳腺珠蛋白;乳腺癌;原核表达系统;蛋白纯化;杂交瘤技术;单克隆抗体;
- prokaryotic expression system 原核表达系统
- Objective:To construct prokaryotic expression systems of ltB/ctB-ompL1/1 fusion genes and 方法:采用连接引物PCR 构建ltB-ompL1/1和ctB-ompL1/1融合基因,常规方法构建其原核表达系统。 采用SDS-PAGE 检测目的重组蛋白rLTB-rOmpL1/1和rCTB-rOmpL1/1表达情况。
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- In the present study, molt-inhibiting hormone-1 from Eriocheir japonica sinensis (Ers-MIH1) was studied both in prokaryotic and eukaryotic expression system. 第二章：中华绒螯蟹蜕皮抑制激素1(Ers-MIH1)基因的原核表达载体的构建
