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- Objective: (1) To establish PCR reaction system that uses allele-specific primer PCR technique to detect SNP of KLOTHO gene. 目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
- The universal primer(CHS1 1S,CHS1 1R)is regarded as a specificity primer. The result showed that the sensitivity of universal primer PCR is 100 fg. 皮肤病原真菌通用引物(CHS1 1S,CHS1 1R)具有较强的特异性,敏感度为100 fg;
- Sensitivity determination of universal primer PCR: Seven samples ranging from 100 ng to 100 fg shown positive,except the 10 fg sample yielding negative band. 皮肤病原真菌通用引物PCR敏感性测定,模板DNA 100 ng~100 fg的7个系列浓度均扩增出了阳性条带,10 fg为阴性。
- Objective To investigate the possibility of applying degenerate oligonucleotide primer PCR(DOP-PCR) and comparative genomic hybridization(CGH) in analysing genomic genetics of a single cell. 目的探索单细胞退变寡核甘酸引物PCR(DOP-PCR)-比较基因组杂交(CGH)技术应用于单细胞全基因组分析及着床前胚胎遗传学研究的可能性。
- Universal Primer PCR for Detection of Clinic Bacteria 通用引物PCR检测临床常见致病菌的实验研究
- Keywords Streptococcus pyogenes;Gene typing;DNA polymorphism;Random primer PCR; 关键词酿脓链球菌;基因分型;DNA多态性;随机引物PCR;
- HBV genotypes were determined by type-specific primers PCR method. PreC/BCP mutations were detected by direct sequencing of PCR products,and Clustal W 1.8 software was applied to analyzing the sequences. 以型特异性引物PCR法检测16对乙肝疫苗阻断失败的母婴和88例阻断成功的母亲血清样本中HBV基因型;用PCR产物直接测序法检测HBV前C/ BCP突变;采用Clustal W 1.;8软件进行序列分析。
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- Application of Pathogenic Bacteria Detection in Surface Water by Universal Primer PCR Method 通用引物PCR方法在地表水病原菌检测中的应用
- Rapid detection of K-ras gene point mutation in pancreatic adenocarcinoma by sequence special primer PCR 顺序特异性引物法快速检测胰腺癌K-ras基因点突变
- The Study of Preparing Probes for Fluorescence in situ Hybridization (FISH) from YAC Clones by Universal Primer PCR 用万能引物PCR法从YAC中分离制备荧光原位杂交探针的研究
- Keywords dermatophytes;fungi identification;animal model;universal primer PCR;AP-PCR; 关键词皮肤病原真菌;真菌检测;动物模型;通用引物PCR;AP-PCR;
- Degenerate oligonucleotide primered PCR 简并寡核苷酸引物PCR
- Keywords Dermatophytes;Fungi identification;Model;animal;Universal primer PCR;AP-PCR; 皮肤病原真菌;真菌检测;模型;动物;通用引物PCR;AP-PCR;
- A coat of primer should be made over the bare surface before the main painting. 在喷涂主漆前,应在物品的表面刷一层底漆。
- Molecular Evidence of a Pair of PCR Primer for Rye 1R Chromosome Specificity ? 黑麦1R染色体特异性PCR引物的分子证据
- I borrowed a primer of science from the library. 我从图书馆借了一本科学的入门书。
- By using these two primer pairs and two times of PCR protocols, Cur. 所以使用这两对引物进行两次PCR反应可将目标菌Cur.
- Keywords Bacillus fastidious uricase;degenerated primer PCR;kinetic uricase method for serum uric acid assay;xanthine interference;gene cloning; 关键词苛求芽孢杆菌尿酸酶;血清尿酸测定专利尿酸酶法;黄嘌呤干扰;简并引物PCR;基因克隆;
- Spray primer to see the condition of surface. 喷水补土检查表面状况。