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- Objective To investigate the effect of tetradrine(Tet) on the contend of MDA,the activity of NOS and Na~+-K~+-ATP,Ca~(2+)-Mg~(2+)-ATP of primary hippocampi neurons cocultured with quinolinic acid(QA). 目的探讨粉防己碱(Tet)对喹啉酸(QA)诱导损伤的原代海马神经元丙二醛(MDA)、一氧化氮合酶(NOS)及Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的影响。
- Method In vitro: primary hippocampus neurons were cultured using embryo rat brains, the MTT and the activity of LDH were assayed and the pathology was observed, so as to study the protection of Tet from excitotoxicity induced by quinolinic acid(QA). 方法:离体实验:采用海马神经元原代培养,检测培养神经元的MTT、 LDH活性,并观察其病理形态学改变,来研究Tet对QA损伤的海马神经元的保护作用。
- Method:In vitro: primary hippocampus neurons were cultured using embryo rat brains, the MTT and the activity of LDH were assayed and thepathology was observed, so as to study the protection of Cin from excitotoxicity induced by quinolinic acid(QA). 方法:采用海马神经元原代培养,检测培养神经元的MTT、 LDH活性,并观察其病理形态学改变,来研究Cin对QA损伤的海马神经元是否有保护作用,利用海马神经元损伤细胞模型,通过检测各组神经元的MDA含量、NOS活性、细胞内游离Ca2+浓度及细胞外几种兴奋性氨基酸和抑制性氨基酸含量的变化情况来探讨Cin对QA损伤海马神经元保护作用的相关作用因素。
- A comparative study of three sorts of nutrient medium on primary hippocampal neurons culture 三种培养液进行海马神经元原代培养的对比研究
- Keywords opioid receptors;CCK-A receptor;CCK-B receptor;morphine dependence;primary hippocampal neurons;laser scanning confocal microscope;flow cytometry; 阿片受体;CCK-A受体;CCK-B受体;吗啡依赖;原代海马神经元;激光共聚焦;流式细胞术;
- primary hippocampal neurons 原代海马神经元
- Conclusions The method for the primary culture of hippocampal neurons is a good method for t... 结论本方法适合一般实验室开展海马神经细胞培养。
- Objective To study topiramate's neuroprotection on primary cultured hippocampal neurons which were injuried by glutamate and its mechanism. 摘要目的探讨托吡酯对离体大鼠海马神经元谷氨酸损伤的保护作用及其机制。
- Objective To search for an easier and cheaper method for the primary culture for hippocampal neurons of SD newborn rats. 目的寻求一种简单、廉价的原代SD乳鼠海马神经细胞培养方法。
- Objective To study the neuroprotective effects of progesterone (PROG) on the injury of primary cultured hippocampal neurons induced by glutamate excitotoxicity. 摘要目的观察孕酮对原代培养海马神经细胞谷氨酸损伤的保护作用。
- Methods The primary culture of SD newborn rats monolayer hippocampal neurons was performed in a simple way,in which hippocampal neurons were digested by trypsin and cultured. 方法采用胰蛋白酶消化法制备游离海马神经细胞悬液,进行培养。
- Objective:To study the ultrastructure of cultured hippocampal neurons in vitro. 目的:研究体外培养海马神经元的表面超微结构。
- Although learning must occur if newborn hippocampal neurons are to survive, not all types of learning work. 虽然必须有学习的刺激才能让新生神经元存活,但并不是所有学习类型都有效。
- In addition, the method using CLSM to investigate the nucleic acidic content of hippocampal neurons was also studied. 此外,本研究还对使用激光扫描共聚焦显微镜测定海马脑片内神经元核酸含量的方法进行了探索。
- Methods The interspike interval (ISI) sequences of discharge of the hippocampal neurons were analyzed. 方法分析长时间顺序记录的猕猴海马神经元自发放电的动作电位间期序列(ISI)。
- Objective To study the early radiobiological response of Gamma knife to the cultured hippocampal neurons in vitro. 目的研究伽玛刀对体外培养的海马神经元的早期放射生物学反应。
- Nimodipine may have protective effects on degenerative hippocampal neurons induced by acute lead poisoning. 尼莫地平可能具有抗急性铅染毒海马神经元损伤的作用。
- Inhibition of Glutamatergic Activation of Extracellular Signal-regulated Protein Kinases in Hippocampal Neurons by the Intravenous Anesthetic Propofol. 静脉麻醉药物异丙酚抑制海马神经细胞的谷氨酸能激活的细胞外信号调节激酶。
- The alteration of the brian with increasing age may be based on the changes of synaptic interface parameters in the hippocampal neurons of mice. 小鼠海马CA_1区神经元突触界面结构参数的变化可能是海马增龄性改变的基础。
- The firing pattern of single hippocampal neuron was obviously modulated both by the ATRC and by the ATPDH. ATRC和ATRDH均能明显调制单个HPC神经元的紧张式放电成为节律性爆发式放电;
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries