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- Objective To establish PCR assay of Porcine Parvovirus detection in Trypsin. 目的建立原材料胰蛋白酶中猪细小病毒的PCR检测方法。
- Objective To develop a PCR method for the determination of porcine parvovirus(PPV). 目的建立猪细小病毒(PPV)PCR检测方法。
- A polymerase chain reaction (PCR) was developed and evaluated to detect porcine parvovirus(PPV). 本研究成功地建立了检测猪细小病毒（PPV）的聚合酶链反应（PCR）技术。
- ObjectiveTo establish the serological method for detection of porcine parvovirus(PPV). 目的建立猪细小病毒血清学检测方法。
- Porcine Parvovirus Infection (PPV) is the most common and important cause of infectious infertility. 猪细小病毒感染是引起猪传染性繁殖障碍的最普遍和最重要的原因。
- A sandwich ELISA for detection of Porcine Parvovirus(PPV) wasdeveloped and applied to detect PPV antigen in fetal tissues. 研究建立了从胎猪脏器中检测猪细小病毒（PPV）抗原的ELISA双抗体夹心法。
- The genomic DNA of porcine parvovirus (PPV) Z strain cultured with PK15 cells was extracted and its NS1 gene was amplified and sequenced. 从猪细小病毒Z株提取基因组DNA ,利用PCR扩增部分基因 ,并对该扩增片段进行测序与分析。
- Inhibitory effects of nitric oxide (NO) on Porcine parvovirus (PPV) infection on porcine kidney (PK-15) cells were studied in the paper. 本文研究了来源于不同前体物的一氧化氮(Nitro oxide, NO)对猪细小病毒(Porcine parvovirus,PPV)体外增殖的影响。
- Different concentrations of selenomethionine on porcine parvovirus(PPV)infection in vitro on the PK-15 cells were studied. 以PK15细胞为模型,研究不同浓度的硒蛋氨酸对猪细小病毒(PPV)体外复制的抑制作用。
- Porcine parvovirus (PPV) causes reproductive failure in swine, manifested as embryonic resorption, fetal mummification, abortion and stillbirths. 猪细小病毒病是由猪细小病毒引起的以初产母猪发生流产、不孕、产死胎、畸形胎、木乃伊胎及弱仔等为特征的猪的主要繁殖障碍性疾病之一。
- Porcine parvovirus (PPV) infection is one of the most important viral diseases, and characterized by reproductive failures in swine. 猪细小病毒(porcine parvovirus，PPV)感染是一种严重危害养猪业的病毒性疾病，广泛分布于世界各地，至今尚未得到有效的控制。 因此研制有效疫苗来防制本病依然是一项十分紧迫的任务。
- Porcine parvovirus(PPV) is one of the major fectors which causes mummification, still births and other reproductive failures in pregnant sows. 猪细小病毒(PPV)是引起母猪畸胎的主要因素之一。
- Porcine Parvovirus, or PPV, is one of the factors that cause pregnant sow abortion, mummy fetus,weak fetus and sterility. 猪细小病毒是引起妊娠母猪繁殖障碍的主要病原之一。 易感染妊娠前期的母猪 ,主要引起母猪流产、胚胎死亡、胎儿畸形、木乃伊、弱胎及不孕等。
- A multiplex polymerase chain reaction was optimized to simultaneously detect classical swine fever virus(CSFV),porcine parvovirus virus(PPV) and porcine pseudorabies virus(PRV). 根据猪瘟病毒 ( CSFV)、猪细小病毒 ( PPV)和猪伪狂犬病病毒 ( PRV) 3种病原体的基因保守序列 ,分别设计了与CSFV的 E2基因、PPV的 VP2基因和 PRV的 g 基因序列互补的 3对引物。
- The serum samples from 407 sew involved PRSD showed positive antibodies to porcine parvovirus(PPV)84.8%,chlamydia 56%,Japanese encephalitis virus 55% and brucella 0%. 在 1071 胎中，共抽样 407 头份血清进行猪细小病毒（ P P V）、衣原体（ Cp）、布氏杆菌（ Br）及乙型脑炎病毒（ J E V）的血清学检查。 其中，猪细小病毒（ P P V）抗体阳性反应高达84.;8%25 。
- Porcine parvovirus(PPV),a major cause of reproductive failure in swine,resulted in early embryonic death,fetal death,still births, and delayed return to estrus. 猪细小病毒（PorcineParvovirus，PPV）是一自主型细小病毒（Autonomousparvovirus），它是引起畸胎的主要因素之一。
- Porcine parvovirus is a fairly tough virus that multiplies normally in the intestine of the pig without causing clinical signs.It is world-wide in its distribution. 猪细小病毒是一种相对顽固的病毒，病毒在猪的肠道内繁殖，但不表现临床症状。
- VP2 Gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced . The results showed that VP2 Gene of Porcine parvovirus (PPV) SD-68 strain contained 1740bp and encoded a protein of 579 amino acids . 对猪细小病毒(PPV)SD-68株VP2基因进行的克隆和序列测定表明:SD-68株VP2基因全长1740bp,编码579个氨基酸残基组成的多肽;
- The porcine parvovirus(PPV)HZ-9 strain isolated in the study was purified from infected porkling kidney cell cultures by high speed centrifugation with polyethylene glycol precipitation and CsCL quilibrirm density centrifugation. 将自行分离鉴定的猪细小病毒 HZ-9株感染仔猪肾原代细胞,采用 PEG 沉淀法及 CsCl密度梯度离心从病毒细胞培养物中提纯病莓。
- The serum antibodies to Porcine Parvovirus(PPV), Japanese B encephalitis virus (JBEV) and Pseudorabies virus(PrV) in serum samples of 67 gilts from two Pig - farms were detected with Latex agglutination test(LAT). 用乳胶凝集试验(LAT)对两个规模化猪场的后备母猪进行猪细小病毒病(PPV)、猪乙型脑炎(JBEV)和猪伪狂犬病(PrV)血清流行病学调查;共检查血清样本67头份;结果检出PPV抗体阳性血清28份;阳性率为41.;79%25; JBEV抗体阳性血清18份;阳性率为26
