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- The rational IVM maturation of porcine oocytes system is NCSU23+20IU/mlPMSG+20IU/mlHCG+10%PFF,of which the maturation rate was 70.3+3.9%. 试验结果得出:最佳的猪卵母细胞培养体系为:NCSU23+20IU/mlPMSG+20IU/mLHCG+10%25PFF;激素作用时间为45h;成熟率可达到70.;3+3
- The effects of PMSG, hCG, E2, rpGH, BSA, NCS, PCS and L-cysteine on the IVM of porcine oocytes were studied in this paper. 本实验就PMSG、hCG、E_2及rpGH对猪卵母细胞体外成熟的影响进行了研究,并比较了BSA、NCS、FCS和L-cysteine在成熟中的作用。
- The IVM rate of porcine oocytes was improved significantly after supplied withboth hCG and PMSG in mature system(P<0.05). hCG(10IU/L)和PMSG(10IU/L)联合使用后成熟率显著高于单独使用PMSG(31.;9%25、17
- The IVM rates of porcine oocytes derived from follicles in diameters 2-3mm, 5-8mmand 3-5mm were increased gradually(12.7%, 31.3%, 46.0%,P<0.05). 小、大、中(2-3mm、5-8mm、3-5mm)三组卵泡卵母细胞的体外成熟率依次为12.;7%25、31
- Three tests have been carried out in this research:test onc showed 3-6mm was suitable diameter of porcine follicular during vitro maturation of porcine oocytes. 本研究对猪卵母细胞的体外成熟和猪颗粒细胞核移植进行了初步探索 :试验一证明 ,体外成熟 4 8h ,来自 3- 6mm大小卵泡的卵母细胞成熟质量较好 ,当卵泡较大或较小时 ,成熟质量较差 ;
- The objective of the present study was to examine the effects of porcine follicular fluid (pFF) and fetal calf serum (FCS) on in vitro maturation of porcine oocytes derived from small follicles (diameter <2 mm). 本研究探讨了猪卵泡液(pFF)和胎牛血清(FCS)对猪小腔卵泡卵母细胞体外成熟的影响。
- The cryopreservation and thawing method of porcine oocytes were reviewed.The effect and influence factors of preservation, choosing protection agents and so on were also discussed. 摘要综述了猪卵母细胞的冷冻保存方法和解冻方法,同时对保存效果、影响保存的因素、保护剂的选择等进行探讨。
- Zona-intact and zona-free porcine oocytes 有无带卵母细胞
- At last, the application foreground of freezing technology of porcine oocyte was prospected. 最后,展望了猪卵母细胞冷冻技术的应用前景。
- The effect of electric field strength pulse number and the IVM oocyte age on the porcine oocyte parthenogenetic activation was studied. 体外成熟40,44,48和52h猪卵母细胞用2次150V/mm,60us电刺激激活,卵母细胞裂解率为0.;0%25、0
- EFFECT OF GONADOTROPHIN ON IN VITRO MATURATION OF PORCINE OOCYTES 促性腺激素对猪卵母细胞体外成熟的影响
- In Vitro Fertilization of Porcine Oocytes and Polyspermy 猪卵母细胞的体外受精及多精受精
- ANP also significantly inhibited FSH-induced porcine oocyte GVBD (47.6% vs. 83.8%) and PB1 emission (22.4% vs. 45.2%) when CEOs were cultured in pFF-free maturation medium. 当猪CEOs培养在无卵泡液的培养液中时;ANP显著抑制FSH诱导的GVBD(47.;6%25vs
- Experiment on Cryopreservation of Porcine Oocytes by Using Glass Micropipette 用玻璃微管法冷冻猪卵母细胞
- Effects of ovary storage temperature on chromatin configuration and in vitro maturation of porcine oocytes 猪卵巢体外保存温度对卵母细胞染色质构型和成熟能力的影响
- STUDIES ON THE INHIBITORY EFFECT OF HYPOXANTHINE ON THE SPONTANEOUS MEIOTIC MATURATION OF PORCINE OOCYTES IN VITRO 次黄嘌呤对猪卵母细胞体外自发成熟抑制作用的研究
- Influence of sequence culture on development competence of parthenogenetic porcine oocytes 序贯培养法对猪孤雌激活胚胎发育能力的影响
- Effect of Different Maturation Time on the in Vitro Fertilization and Parthenogenesis of Porcine Oocytes 不同成熟时间的猪卵母细胞对体外受精的影响及孤雌发育
- Effect of FSH and LH on In Vitro Maturation of Porcine Oocytes and Different Maturation Times on Enucleation Efficiency 对猪卵母细胞核成熟及体外成熟时间对去核效率的影响
- Effect of Follicle-stimulating Hormone and Luteinizing Hormone on In Vitro Maturation of Porcine Oocytes 促卵泡素与促黄体素对猪卵母细胞体外成熟的影响