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- The factors influencing survival rate of LAB and plasmid stability during lyophilization were systematically studied. 对乳酸菌冻干存活率的影响因素及质粒稳定性进行研究。
- By the measurement of plasmid-bearing cells, it was shown experimentally that the increase of antibody expression was due to the improvement of plasmid stability. 通过测定发酵过程中质粒的稳定性,证明抗原产量的提高是由于质粒稳定性提高所致。
- Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor. 发酵过程中维持低葡萄糖水平可以限制细胞的生长速率,提高质粒稳定性和促进青霉素G酰化酶的合成。
- The glucose concentration was controlled around 0.1g/L and the average specific growth rate was 0.06h~(-1). Under the above conditions the plasmid stability reached 86% and penicillin G acylase activity was 23 000U/L. h恒速流加葡萄糖至35h;控制流加过程葡萄糖浓度0.;1g/L左右;平均比生长速率为0
- Since constitutive expression system is more likely leading to the loss of plasmid compared with inducible system,enhancing the plasmid stability of a constitutive system is more difficult. 与诱导型表达系统相比,在组成型表达系统中,由于外源蛋白的持续表达,导致细胞代谢负担加重,质粒稳定性的控制更加困难。
- Optimization of expression of double genes co-expression vector and plasmid stability 双基因共表达载体的诱导表达优化及质粒稳定性
- Global strategic balance and stability. 全球战略平衡和稳定。
- Keywords Salmonella;type I fimbriae;dendritic cells;LD50;invasion;plasmid stability; 沙门氏菌;I型菌毛;树突状细胞;LD_(50);体内侵袭试验;质粒稳定性;
- Keywords penicillin G acylase;constitutive expression system;plasmid stability;glucose; 青霉素G酰化酶;组成型表达系统;质粒稳定性;葡萄糖;
- Keywords recombinant human aFGF mutant;plasmid stability;Escherichia coli;induction;restriction digestion graph; 重组人酸性成纤维细胞生长因子改构体;质粒稳定性;大肠杆菌;诱导;酶切图谱;
- Keywords L\|asparaginase;Engineered strain;Culture conditions;Plasmid stability; 天冬酰胺酶;工程菌;培养条件;质粒稳定性;
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- Keywords Saccharomyces cerevisiae;expression system;homologous recombination;yeast episomal plasmid;plasmid stability;plasmid copy number;fusion protein; 酿酒酵母;表达系统;同源重组;附加体型载体;质粒稳定性;质粒拷贝数;融合蛋白;
- Political stability is essential to economic prosperity. 政治稳定对经济繁荣是必要的。
- Something that gives stability, especially in character. 稳定因素表明稳定性的因素,尤指在性格方面
- This is conducive to economic stability. 这有助于经济的稳定。
- Recessing the oxidizer tube improves stability. 氧化剂管的凹陷使稳定性增强。
- The government has taken a measure to maintain the stability of prices. 政府已经采取了措施以确保物价稳定。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.