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- P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III. 扩增p 16 promoter一luc一pGLZ一Basic报告基因表达质粒并提取其DNA。
- plasmid amplification 质粒扩增
- Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification. 用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA,直接转化大肠杆菌。
- Some of them require further amplification. 其中有些内容需进一步详细阐明。
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- That comment needs some amplification. 这个评论需要一些阐明。
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- You look at the nine times amplification? 你再放大九倍看看?
- Amplification won't double Icicle's damage. 扩增不会双重冰柱的损害。
- A few remarks added in amplification and defense. 一些注释加到详叙和辩护中。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- Objective To construct the plasmid expressing BDV p40 gene. 目的构建博尔纳病病毒p40基因重组表达质粒。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- Method: PCR amplification,DNA sequencing and cladistic analysis. 方法:PCR扩增、DNA序列测定、分支分析。
- The PNL-BDNF-IRES2-EGFP plasmid was identified to be correct. 构建的慢病毒载体质粒PNL-BDNF-IRES2-EGFP经Sal I和BamH I双酶切鉴定正确,三质粒共转染293T细胞后荧光激发可见大量绿色荧光。
- The voice of despair may be weak and need amplification. 绝望的呼声可能很微弱,需要变大。
- ASE of dye medium in a DLA and fs pulse amplification[J]. 引用该论文 肖纲要;何慧娟;沈晋汇;刘玉璞;张影华;陆培华;王之江.
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
- I focus on the role of antisense RNAs in plasmid replication. 本文着重叙述反义RNA在质粒复制中的作用及其进展。