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- plasmid DNA vector 质粒DNA载体
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
- With Southern Blotting hybridization of R plasmid DNA with gentamycin resistant gene probe from America, Australia and our lab,a different gentamycin resistant gene was found and thd new gene was cloned into pBluescript SK( I ) vector. 应用源自美国、澳洲及本室的庆大霉素耐药基因探针对分离的庆大霉素耐药菌株进行Southern印迹杂交,又发现了另一种与之不同源的庆大霉素耐药基因,并对该基因进行了分子克隆和初步分析。
- Objective To develop a procedure for large-scale purification of plasmid DNA. 摘要目的建立适合大规模纯化质粒DNA的工艺。
- Experiments demonstrated that the DNA vector inhibited the replication of BmNPV both in vivo and in vitro. 研究结果表明，该载体DNA在体外、体内均能较好地抑制家蚕核型多角体病毒在细胞中的复制。
- P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III. 扩增p 16 promoter一luc一pGLZ一Basic报告基因表达质粒并提取其DNA。
- This arti cle reviews the structure of plasmid DNA, especially the structure of its Assist ant units and effects. 本文阐述了基因免疫中质粒DNA中的结构组成特别是其佐剂单位及其作用。
- Plasmid DNA and calf-DNA are irradiate d by laser with the samestrengty and different irradiation period. 激光作用质粒DNA和小牛胸腺DNA产生损伤效应，导致DNA结构变化。
- The traditional alkaline method to isolate plasmid DNA takes time and the operation system requires strict control. 实验室中常用的质粒提取方法是碱裂解法。
- Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material). 开启真空装置使液体通过管柱(质体DNA会与管柱物质结合)。
- Ion exchange HPLC is an alternative that yields milligram quantities of purified plasmid DNA within an hour. 离子交换HPLC作为替代方法可以在一小时内生产毫克量的纯化的质粒DNA。
- Routine alkaline lysis miniprep of plasmid DNA was modified,and LiCl was specially used. 对碱裂质粒小量法进行了改进，并且在提取过程中增加了LiCl处理。
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
- Methods Plasmid DNA was extracted by the procedure of Kado and Liu,and Pgm was detected by incubating isolates on Congo red media at28oC for7days. 方法质粒运用Kado改良法进行提取; Pgm利用刚果红培养基置28℃培养7天观察。
- A variety of approaches have been taken to augment and modulate the immunogenicity and efficacy of plasmid DNA vaccines. 核酸疫苗虽然在小动物实验中取得成功,但对大动物、灵长类动物和人类的免疫原性却很低。
- Assuming that the plasmid DNA is endotoxin-free, mention how you think "danger" is signaled by DNA of bacterial or plasmid origin (5 points). 假设这个质体DNA不含内毒素，提出理由说明你认为"危险"讯息是源自细菌的DNA或是质体（5分）。
- The damage effect of plasmid DNA and mutation induced of lacZ gene were analysesed by low power HeNe laser radiation invitro plasmid DNA. 利用低功率HeNe激光辐照离体质粒DNA，分析了质粒DNA的损伤效应和lacZ基因的突变。
- Luciferase plasmid DNA and PEI were complexed to form slightly positive-charged nanoparticles, onto which FOL-PEG-PLL conjugate was surface coated. 首先用荧光素酶质粒DNA和PEI合成略带正电的纳米颗粒，再在其表面包裹FOL-PEG-PLL共轭化合物。
- Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification. 用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA，直接转化大肠杆菌。
- The money will be used to build up a state-of-the-art cGMP level manufacturing plant for vaccine production and plasmid DNA contract manufacturing business. 国光生技公司在历届经理人稳健经营的领导下，本著以开发生产疫苗为职志，为维护人类健康而努力。
