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- plasmid DNA extraction 质粒DNA提取
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
- Methods: The improved CTAB DNA extraction method. 方法:改良CTAB法。
- Methods Agarose gel electrophoresis of plasmid DNA, adherence test, and extract of plasmid were used.Electron microscope photos were prepared and observed. 方法:应用琼脂糖凝胶电泳试验、电镜照片制备及观察、粘附试验及质粒消除试验。
- Objective To develop a procedure for large-scale purification of plasmid DNA. 摘要目的建立适合大规模纯化质粒DNA的工艺。
- Genomic DNA Extraction from the Polygonum multiflorum Thunb. 中药何首乌总基因组DNA的提取。
- P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III. 扩增p 16 promoter一luc一pGLZ一Basic报告基因表达质粒并提取其DNA。
- Study on Genomic DNA Extraction from Recalcitrant Dimocarpus longan Lour. 顽拗植物龙眼基因组DNA提取方法的研究。
- This arti cle reviews the structure of plasmid DNA, especially the structure of its Assist ant units and effects. 本文阐述了基因免疫中质粒DNA中的结构组成特别是其佐剂单位及其作用。
- Plasmid DNA and calf-DNA are irradiate d by laser with the samestrengty and different irradiation period. 激光作用质粒DNA和小牛胸腺DNA产生损伤效应,导致DNA结构变化。
- The quality of DNA by different methods of genome DNA extraction was different. 采用不同方法提取的3种松科植物叶片基因组DNA的质量差异较大。
- The traditional alkaline method to isolate plasmid DNA takes time and the operation system requires strict control. 实验室中常用的质粒提取方法是碱裂解法。
- A modified CTAB method of genomic DNA extraction of Aquilaria sinensis(Lour. 在传统CTAB法的基础上加以改良,建立了一种适于白木香基因组DNA提取的方法。
- Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material). 开启真空装置使液体通过管柱(质体DNA会与管柱物质结合)。
- Ion exchange HPLC is an alternative that yields milligram quantities of purified plasmid DNA within an hour. 离子交换HPLC作为替代方法可以在一小时内生产毫克量的纯化的质粒DNA。
- Routine alkaline lysis miniprep of plasmid DNA was modified,and LiCl was specially used. 对碱裂质粒小量法进行了改进,并且在提取过程中增加了LiCl处理。
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
- Methods Plasmid DNA was extracted by the procedure of Kado and Liu,and Pgm was detected by incubating isolates on Congo red media at28oC for7days. 方法质粒运用Kado改良法进行提取; Pgm利用刚果红培养基置28℃培养7天观察。
- Results showed that GMO DNA extraction method was better than CTAB method, but both DNA template were suitable for PCR amplification. 结果表明:GMO试剂盒提取法和改良CTAB两种方法的DNA质量无明显差异,采用CTAB法所提取DNA质量较好,能够满足进一步PCR扩增的需要。
- A variety of approaches have been taken to augment and modulate the immunogenicity and efficacy of plasmid DNA vaccines. 核酸疫苗虽然在小动物实验中取得成功,但对大动物、灵长类动物和人类的免疫原性却很低。