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- Hie resultant vector was checked by expression of phage antibody, ELBA and in vivo recombination. 通过噬菌体抗体的表达、ELISA及细胞内重组试验鉴定所获得的表达载体。
- AIM: To construct a anti dodecane tertraacetic acid yttrium (DOTA Y) immune Fab phage antibody library. 目的 :构建钇 十二烷四乙酸 (Y DOTA)免疫噬菌体Fab抗体库。
- We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library. 为了构建人源性抗脑胶质瘤抗体库，我们必须先从患者的血液内检测到抗脑胶质瘤抗体，才能为建立人源性噬菌体抗体库奠定基础。
- AIM: To construct the phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus. 目的 :利用抗SARS冠状病毒IgG抗体阳性的SARS康复患者外周血淋巴细胞 ,构建人源Fab段抗体文库。
- The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds.The last round enriched phage clones were used to reinfect. 由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。
- Objective: To construct human tetanus immune phage antibody library, screen antibody genes against tetanus toxoid, and express solution Fab in Escherichia coli. 目的:建立人破伤风免疫噬菌体抗体库,筛选抗破伤风类毒素的抗体基因,并在大肠杆菌中表达可溶性Fab段。
- Methods By genetic engineering technology,human anti-keratin ScFv was constructed from Fab fragment selected from established semi-synthetic phage antibody library. 方法利用基因重组技术对从半合成噬菌体抗体库中克隆的人源性抗角蛋白抗体Fab片段进行改造,获得了人源性抗角蛋白ScFv片段,并进行DNA序列分析。
- Phage antibody chips, prepared by coupling the recombinant phage displaying scFv with carboxyl-terminated silicon, can be applied in detecting proteins and proteomes. 摘要将展示单链抗体的重组噬菌体与羧基终止的矽片偶联，制成噬菌体抗体芯片，可用于检测多类蛋白质和蛋白质组。
- The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology. 噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法,在生物科学领域极具潜力。
- Wang Y, Hua B, Liu QY, et al.By-passing immunization: Construction of a phage antibody library with randomized CD3[J].ChinJ Microbiol Immunol, 1997, 17(6): 449-52. [5]王琰;化冰;刘群英;等.;随机化CD3抗体库的构建及不经免疫制备抗体的初步探讨[J]
- Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research. 结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体,为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
- Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application. 结论制备的可溶性抗Fas?Fab抗体具有抗Fas抗体的特异性,为进一步的研究奠定了基础。
- We successfully constructed the Fab phage antibody library of mouse male specific antigen, these new molecules are potentially valuable tools in the study of H-Y antibody and the application of sex embryos. 以上结果表明：本研究成功构建了针对血清学检测到雄性特异性抗原的鼠源性噬菌体抗体库，为进一步筛选高亲和力的噬菌体抗体、提高基因工程抗体的亲和力、高效表达可溶性Fab抗体等研究奠定了坚实的实验基础。
- In the present paper the application of DNA library, phage display random peptide library, phage antibody library, etc. in the research of serodiagnostic reagents and their special values were reviewed. 本文综述了基因文库、噬菌体展示肽库及噬菌体抗体库技术在血清学诊断试剂研制中的应用及其各自的优点。
- Our previous studies have screened a clone of human anti-keratin antibody (FK315) from a semisynthetic phage antibody library, and successfully obtained its soluble expressing Fab fragments. 有鉴于此，我们己自半合成噬菌体抗体库中筛选、克隆了一株人源性抗角蛋白抗体FK3，并成功得到可溶性表达的 Fab段。
- After mice were immunized with BoNTB/Hc, the spleens were harvested ,and single-chain Fv (scFv) and Fab phage antibody libraries were constructed from the immunoglobulin heavy and light chain variable region genes. 用重组表达并纯化的BoNTB/Hc免疫小鼠;从其脾淋巴细胞中提取全套抗体可变区基因;分别构建scFv和Fab段噬菌体抗体文库;库容分别为6.;72x10~7cfu和5
- Abstract：Objective: To clone human anti-keratin antibodies from semisynthetic phage antibody library. 摘 要：目的： 从半合成噬菌体抗体库中筛选人源性抗角蛋白抗体并进行鉴定。
- Zhang GF, Wang Y, Wang YM, et al.Cloning of an ti-breast cancer monoclonal antibodies from phage antibody libraries [J].Chin J Oncol. 1995, 17(4): 258-63. [6]张广发;王琰;王雅明;等.;噬菌体抗体库的构建及抗肿瘤单抗的筛选[J]
- High-density phage antibody chip 高密度噬菌体抗体芯片
- Phage antibody library technology 噬菌体抗体库技术
