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- Human immunoglobulin combinatorial library was generated by using phage surface display expression system, and phage antibodies (Fab fragments ) to hepatitis B surface antigen (HBsAg )were screened from it. 应用噬菌体表面递呈表达系统构建了人抗体组合文库,并筛选出了结合乙肝表面抗原(HBsAg)的人噬菌体抗体(Fab片段)。
- The ScFv gene was expressed as fusion protein with phage g3p coat protein and displayed on the phage surface. 结果通过免疫荧光鉴定,这种抗体仍保留着亲代抗体的特性,能与登革3型病毒发生特异性结合。
- M13 bacteriophage has been extensively applied in phage surface display technique. M13噬菌体已被广泛地应用于噬菌体展示技术.
- The techniqu e of GEAs such as ribosome display and phage surface display and their applicati on progress were summarized in this review. 本文将介绍核糖体展示、噬菌体表面展示等基因工程抗体技术及应用进展。
- The phage display antibody library is developed by reasonably combining the antibody library technology and phage surface display system. 将抗体库技术与噬菌体表面展示系统有机地结合就发展成为噬菌体展示抗体库。
- AIM: To construct a human recombinant immunoglobulin library containing D-Dimer by using phage surface display technology. 目的:应用噬菌体展示技术构建人源性抗D二聚体噬菌体抗体抗体组合文库。
- Objective To determine the surface expression of Fas Ag on RBL 2H3 and its function. 目的 研究大鼠肥大细胞表面Fas的表达情况及其功能。
- Utilizing the technology of phage surface display, special antibody can be gained from the human naive Fab phage display library, which can be used as a new therapy for tumors. 本研究构建的人天然Fab段噬菌体抗体库可为进一步筛选特异性抗体,用于免疫治疗打下基础。
- To determine the surface expression of Fas Ag on rat mast cells and its function. 明确大鼠肥大细胞表面Fas表达及其功能;
- The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology. 噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法,在生物科学领域极具潜力。
- Furthermore, by examining CD71 surface expression, we are able to distinguish and differentially isolate anergic and actiated T cells in io. 另外;通过检测CD71的胞膜表达;我们能够在体内鉴别并分离无能与活化的T细胞.
- Alternatiely, the induction of anergy results in markedly less S6 kinase 1 Thr(421)/Ser(424) phosphorylation and CD71 surface expression. 换之;无能可导致明显地S6激酶1在421位苏氨酸/424位丝氨酸磷酸化和CD71胞膜表达的减少.
- The field is located on a large, gentle WNW-plunging anticline with surface expression, that is cut by numerous NE-trending normal faults. 两个狭窄的地堑将储层分为三个气藏,主要的一个气藏位于海滨和滨外;
- Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application. 结论制备的可溶性抗Fas?Fab抗体具有抗Fas抗体的特异性,为进一步的研究奠定了基础。
- Background: Platelets stimulate coagulation via surface expression of phosphatidylserine (PS), providing a negatively charged surface for thrombin generation. 背景:凝血通过刺激血小板表面表达的磷脂酰丝氨酸( PS部门) ,提供了一个负电荷的表面凝血酶生成。
- The results indicated that the enchancement of HLA-I heavy chain synthesis and TAPi gene mRNA expression in HBV transfected cells might mediate the up-regulation of HLA-I surface expression. 提示HBV诱导转染细胞内HLA一A基因重链合成量及1人P:基因转录水平增高,可上调细胞的HLA一I表面表达。
- Methods: Changes of platelet surface expression of CD41/CD42a, CD36/TSP, CD62/CD63, CD9 and CD31 were measured before and after FLP treatment by using flow cytometer in 30 cases of mid late stage lung cancer. 方法 :采用流式细胞技术检测 30例中晚期肺癌患者服肺瘤平二号前后血小板表面糖蛋白CD4 1/CD4 2a、CD36 /TSP、CD6 2 /CD6 3、CD9、CD31表达的改变 ,并与 50名健康志愿者的检测结果进行对照。
- Transfection of ovarian carcinoma cells with the eukaryotic vector pcDNA3 Fas enhanced cell surface expression of Fas gene and induced susceptibility to Fas antibody mediated apoptosis. 经 pcDNA3 Fas真核表达载体转染的卵巢癌细胞Fas基因表达及对Fas单抗诱导凋亡的敏感性均有不同程度提高。
- According to work by Geoffrey Collins of Wheaton College and Jason Goodman of the Woods Hole Oceanographic Institution, this basin could be the surface expression of a subsurface sea. 根据美国惠顿学院的柯林斯(GeoffreyCollins)和伍兹赫尔海洋研究所的古德曼(JasonGoodman)的研究结果,这个盆地可能是地下海洋存在的徵兆。
- bacteriophage surface expression system 噬菌体表面表达系统