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- penioillin acylase 青霉素酰基转移酶, 青霉素酰化酶
- The optimum pH was 7.0 for GL-7-ACA acylase. GL-7-ACA酰化酶反应最适pH为7.;0。
- Secretory Expression of GL?7?ACA Acylase in ?E.coli? GL-7-ACA酰化酶在大肠杆菌中的分泌表达
- Localization of activity of immobilized penicillin G acylase was studied by X ray microanalysis. 利用X射线微区分析,对固定化青霉素G酰化酶的活性进行了定位分析。
- A novel method for rapidly obtaining the GL-7-ACA acylase genes from the soil was presented. 提出了一条快速获取GL-7-ACA酰化酶基因的新方法。
- Activity andstability of immobilized glucoamylase and penicillin acylase were studied. 本文着重论述了两大类固定化酶载体的合成及其分别用于固定糖化型淀粉酶和青霉素酰化酶的研究。
- The genomic DNA was extracted from the selective cultures and the GL-7-ACA acylase gene was directly amplified by PCR technique. 从土壤的选择性培养液中提取微生物的DNA，通过PCR的方法直接扩增成功得到GL-7-ACA酰化酶基因。
- This paper reviews the recent research and application of the support material for the immobilization of penicillin acylase(PA). 就青霉素酰化酶固定化载体材料的研究和应用进行了论述。
- The modified penicillin acylase was mainly present in the PEG phase in a PEG-dextran aqueous two-phase system, and the partition coefficient was 25. 酶在带修饰酶的聚乙二醇与葡聚糖组成的两水相体系中的分配系数为25。
- In this paper,the culture conditions for extracellular penicllin acylase (PA)produced bya strain of Bacillus megaterium K 39 were reported. 该文对一株巨大芽孢杆菌产胞外青霉素酰化酶的发酵条件进行了初步研究。
- This paper reviews the recent research and application of the support material for the immobilization of penicillin acylase (PA). 摘要就青黴素酰化酶固定化载体材料的研究和应用进行了论述。
- Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor. 发酵过程中维持低葡萄糖水平可以限制细胞的生长速率,提高质粒稳定性和促进青霉素G酰化酶的合成。
- The expressed Vitreoscilla hemoglobin (VHb) in engineered Escherichia cole of IL-2, TNF and penicillin acylase could improve the cell growth and desired products in the oxygen-limited condition. vgb基因的表达产物(Vitreoscilla Hemoglogin,VHb)可促进青霉素酰化酶和TNF、IL-2等基因工程菌在低氧条件下细胞生长和产物表达的状况,由于vgb基因的表达降低了细胞对氧的敏感程度,可望运用它来改善发酵过程中溶氧控制裕度。
- When the rate of adsorption of GL-7-ACA acylase on prepared support was compared with those of on commercial ones, it is correspond to TALON metal resin and FP-IDA, and 1.5 folds to EC-IDA. 其中;固定化戊二酰-7-氨基烷酸酰化酶酶活和固定化在TALON 金属树脂、Sepabeads FP-IDA 的相当;是Sepabeads EC-IDA 的1.;5 倍。
- The glucose concentration was controlled around 0.1g/L and the average specific growth rate was 0.06h~(-1). Under the above conditions the plasmid stability reached 86% and penicillin G acylase activity was 23 000U/L. h恒速流加葡萄糖至35h;控制流加过程葡萄糖浓度0.;1g/L左右;平均比生长速率为0
- immobilized penicillin G acylase 固定化青霉素酰化酶
- glutaryl-7-amidocephalosporanic acid acylase 戊二酰基-7-氨基头孢烷酸酰化酶
- Study on Immobilization of Acylase with Cellulose Acetate Membrane 醋酸纤维素固定化酰化酶膜的研究
- Penicillin Acylase Extraction by Osmotic Shock Method 用渗透压冲击法提取青霉素酰化酶
- IMMOBILIZATION OF PENICILIN G ACYLASE ON POLYACRYLONITRILE FIBER 以聚丙烯腈纤维为载体制备固定化青霉素G酰化酶的研究
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries
