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- "It requires a lot of know-how and hand-eye coordination," Porterfield said of patch clamping. 当离子通过一个细胞的隔膜时，离子通道和泵会产生一个不同的电压，细胞通过这种方式制造能量转移电信号。
- The system can be used in conjunction with patch clamp and iontophoresis, as well as our visualization protocols. 此套系统可以与膜片电位钳制法、离子电泳法、以及视觉显影的型式做结合。
- Abstract: The study of lens cells by patch clamp technique began in th e early 90"s. 文摘:应用膜片钳技术对晶体细胞的研究始于90年代初。
- Methods Whole cell patch clamp techniques were used to investigate the effects of 2-APB on Isoc in human hepatocytes. 方法应用全细胞膜片钳记录技术测定人肝细胞Isoc,并观察2-APB对该电流的影响。
- Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent. 方法 :采用全细胞膜片钳技术 ,记录钠通道电流。
- Applying iontophoretic pulses while patch clamping the postsynaptic cell then yields a functional description of each synapse's strength. 当电极贴片夹到突触后细胞时，利用离子电流脉冲来产生每一个突触强度的功能性描述。
- Method Ion currents of rabbit ventricular myocyte were recorded using the whole cell patch clamp technique. 方法应用全细胞膜片钳技术记录兔心室肌单个细胞的电流。
- Methods Whole cell patch clamp technique was used to test the effects of Pho and Fen on TTX-S and TTX-R sodium currents in DRG neurons. 方法应用膜片钳技术研究氰戊菊酯和辛硫磷对DRG河豚毒素敏感（TTX-S）和不敏感（TTX-R）型钠通道的作用。
- Action of ion channel on mem brane is the key events of messenger transduction for excitable cells,which can be detected by the patch clamp technique. 离子通道的活动是可兴奋细胞传递信息的基础,膜片钳技术的发明导致了生命科学的一场革命。
- Peak currents were recorded in HEK293 cells treated by rocuronium, vecuronium, atracurium individually using whole cell patch clamp technique. 分别研究罗库溴铵、维库溴铵和阿曲库铵各药对两种受体亚型的作用。
- Objective: To study ion channel character of the human hepatocyte carcinoma cells of cell line BEL?7402. Methods: The whole?cell recording mode of patch clamp technique. 目的：研究人肝癌细胞系BEL?7402细胞膜离子通道特性。
- Methods Whole cell patch clamp techniques were employed to investigate the effect of aminophylline on L-type calcium cur-rent of diaphragmatic muscles in rats. 方法:采用全细胞膜片钳（whole cell patchclamp）技术;当维持电位为-80mv;刺激频率为0.;5Hz;钳制时间为300ms;步进电压为10mv;去极到60mv;观察氨茶碱对膈肌细胞L型钙通道（L-ICa）的影响。
- Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel. 膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
- Methods Electrophysiology whole cell patch clamp technique was employed to record the electrical activity of fourty amygdaloid nucleus in rat slice preparations. 方法在40例小鼠脑薄片标本上对杏仁核簇神经元进行电生理全细胞膜片箝记录。
- ABSTRACT：Objective To investigate the method of stably isolating pachyntic Spraque Dawley rat calcium tolerant ventricular myocytes for patch clamp techniques. 随着心肌细胞电生理和分子生物学研究的兴起,成熟单个心肌细胞越来越多的应用于心血管疾病病理生理的研究。
- Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY). 实验利用全细胞膜片钳技术研究了荧光染料核黄(NY)标记的支配牙髓的痛感觉神经元的电生理特征。
- Methods Passaged cells were plated on plastic coverslips.Resting membrane potential (RMP), action potential (AP) and input resistance were recorded by whole cell patch clamp. 方法传代细胞单层贴壁培养,细胞内电流钳记录静息膜电位、动作电位、膜阻抗等电生理指标。
- The effect of a general anaesthetic (halothane) on the muscarinic K+ channel (iK,ACh) in guinea-pig atrial cells was investigated using the whole-cell patch clamp technique. 本实验采用全细胞膜片箝方法, 观察了麻醉药氟烷对豚鼠心房肌细胞iK, ACh的影响。
- The anion currents elicited by swelling rat ventricular myocytes in 1T and 0.6T solution, namely ICl,swell were studied using patch clamp techniques with whole - cell voltage clamp configuration. 采用全细胞电压钳方式记录大鼠心室肌细胞在1T及0.;6T溶液中ICl;swell的变化。
- The cell type A and cell type B were immunoactive to anti-CHH and anti-MIH serum respectively. In the present paper,the whole-cell patch clamp technique was used to examine the effects of 5-HT on the excitability and neurosecretion of MTXO cells. 在电流钳模式下,5-HT使河蟹眼柄MTXO A型和B型细胞产生去极化反应,并诱导细胞兴奋,出现不同频率的动作电位;
