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- One of the packaging cell lines was positive for mycoplasma.No RCR was detected in all of the packaging cell lines. 结果 在所建立的靶基因包装细胞系中,无菌试验均为阴性,一株包装细胞支原体呈阳性,全部包装细胞系未测到可复制性逆转录病毒。
- Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent. 通过脂质体转染技术将重组逆转录病毒载体(pMX-3FLAG-Id3-EGFP)转染Phoenix-ecotropic包装细胞系;
- Common toothbrush packaging cells and the use of suction blister card. 常见的电池和牙刷包装多用吸卡泡壳。
- Then pLHCX/TH and pLNCX_2/AADC were transfected into packaging cell line PA317 with liposome. PA317/TH andPA317/AADC were selected by different antibiotics. Gene expression was examined by methods of immuno-histochemistry and in situ hybridization. 我们分别构建了重组逆转录病毒载体pLHCX/TH及pLNCX_2/AADC,通过脂质体介导将带有目的基因的载体分别转到包装细胞PA317中,经筛选得到产病毒的细胞PA317/TH和PA317/AADC,采用免疫组化、原位杂交方法检测目的基因表达;
- helper [virus] free packaging cell 无辅助病毒包装细胞
- helper virus free packaging cell 无辅助病毒包装细胞
- helper[virus] free packaging cell 无辅助病毒包装细胞
- helper[virus]free packaging cell 无辅助病毒包装细胞
- Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs. 反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。
- A storage cell in some amorphous memory devices. 某些非晶体存储器中的一个存储单元。
- She was found prostrate on the floor of the cell. 有人发现她趴在小屋的地板上。
- The prisoner rapped out a message on the cell wall. 那囚犯敲打囚室的墙来传达讯息。
- Keywords analgesia;enkephalin;retrovirus vector;packaging cell line; 镇痛;脑啡肽;逆转录病毒载体;包装细胞系;
- The prisoner raped out a message on the cell wall. 那囚犯敲打囚室的墙来传达讯息。
- The growth of successive layers of a cell wall. 敷着,附着细胞壁连续层的生长
- The special packaging will run up the price of the item. 这种特殊包装会抬高这种商品的价值。
- The prisoner was locked in a cell. 那囚犯被关在单人牢房内。
- The prisoner paced the floor of his cell. 那犯人在牢房里来回地走著。
- The stage was designed to imitate a prison cell. 舞台设计成牢房的样子。
- The prisoner clawed at the cell door in desperation. 囚犯绝望地乱抓牢房的门。