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- Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs. 反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。
- Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent. 通过脂质体转染技术将重组逆转录病毒载体(pMX-3FLAG-Id3-EGFP)转染Phoenix-ecotropic包装细胞系;
- Then pLHCX/TH and pLNCX_2/AADC were transfected into packaging cell line PA317 with liposome. PA317/TH andPA317/AADC were selected by different antibiotics. Gene expression was examined by methods of immuno-histochemistry and in situ hybridization. 我们分别构建了重组逆转录病毒载体pLHCX/TH及pLNCX_2/AADC,通过脂质体介导将带有目的基因的载体分别转到包装细胞PA317中,经筛选得到产病毒的细胞PA317/TH和PA317/AADC,采用免疫组化、原位杂交方法检测目的基因表达;
- Optimized methods for preparation of viron producing package cell line 产生重组逆转录病毒包装细胞系建立及病毒滴度影响因素
- One of the packaging cell lines was positive for mycoplasma.No RCR was detected in all of the packaging cell lines. 结果 在所建立的靶基因包装细胞系中，无菌试验均为阴性，一株包装细胞支原体呈阳性，全部包装细胞系未测到可复制性逆转录病毒。
- Construction of mIL-12 recombinant retrovirus vector and establishment of viral package cell line mIL-12重组逆转录病毒载体的构建及包装细胞系的建立
- Construction and identification of a retroviral vector containing GK gene and its package cell line 葡萄糖激酶基因逆转录病毒表达载体及包装细胞系的构建及鉴定
- package cell line 包装细胞系
- Maintain the cell line equipments. 负责尚能电池线设备维护。
- We have obtained a stable UT-A2-FRT cell line. 经BSD筛选21 d后得到稳定表达UT-A2的细胞株;
- Materials1) Bel7402 cell line and COS? 肝癌Bel7402细胞系和COS7细胞系
- HL60/DNR is a model cell line of MDR1. HL60/DNR为典型的表达MDR1的细胞株
- Keywords analgesia;enkephalin;retrovirus vector;packaging cell line; 镇痛;脑啡肽;逆转录病毒载体;包装细胞系;
- Objective To study the effect of SKI-2053R on SGC-7901 cell line. 目的 :研究SKI 2 0 5 3R对胃癌细胞株SGC 790 1的作用及其机制。
- We obtained the similar results both on BMDC and DC2.4 cell line. 在树突状细胞株DC2.;4细胞表面也得到相似的结果。
- Transfection, make stable C/EBP p expression cell line. 质粒转染，制备稳定表达C尼BPp的细胞系。
- Results A fusion cell line,FLB2C,was obtained through screening. 结果经筛选获得一株融合细胞FLB2C。
- There are two types of cell lines. 有两种类型的细胞系。
- The whole FHIT gene was found deleted in an EC cell line, EC8733. 在食管癌细胞系EC8733中发现了FHIT全基因的丢失。
- OPG was expressed in BMA-1 cell lines. MBA- 1细胞表达OPG ;
