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- Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs. 反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。
- One of the packaging cell lines was positive for mycoplasma.No RCR was detected in all of the packaging cell lines. 结果 在所建立的靶基因包装细胞系中,无菌试验均为阴性,一株包装细胞支原体呈阳性,全部包装细胞系未测到可复制性逆转录病毒。
- Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent. 通过脂质体转染技术将重组逆转录病毒载体(pMX-3FLAG-Id3-EGFP)转染Phoenix-ecotropic包装细胞系;
- The gay package is only for dandy. 这种鲜艳的包装只是为了美观。
- The goods are to be sent in airtight package. 这批货物应密封包装运送。
- Common toothbrush packaging cells and the use of suction blister card. 常见的电池和牙刷包装多用吸卡泡壳。
- My aunt is hooked on package holidays in Spain. 我的姑母着迷于旅行社包办的西班牙度假。
- Then pLHCX/TH and pLNCX_2/AADC were transfected into packaging cell line PA317 with liposome. PA317/TH andPA317/AADC were selected by different antibiotics. Gene expression was examined by methods of immuno-histochemistry and in situ hybridization. 我们分别构建了重组逆转录病毒载体pLHCX/TH及pLNCX_2/AADC,通过脂质体介导将带有目的基因的载体分别转到包装细胞PA317中,经筛选得到产病毒的细胞PA317/TH和PA317/AADC,采用免疫组化、原位杂交方法检测目的基因表达;
- The package carries no written message. 包裹内不得夹带书信。
- The proposal was presented as final package deal. 那项建议是作为最终的一揽子交易提出来的。
- He tied the package with a cord. 他用绳子把包裹扎紧。
- Can you deliver a large package of books? 你能把一大包书寄出去吗?
- A storage cell in some amorphous memory devices. 某些非晶体存储器中的一个存储单元。
- We tied up both package well with heavy cord. 我们用粗绳把所有包裹捆扎妥当。
- She was found prostrate on the floor of the cell. 有人发现她趴在小屋的地板上。
- The package arrived safe and sound. 包裹寄到,丝毫无损。
- The prisoner rapped out a message on the cell wall. 那囚犯敲打囚室的墙来传达讯息。
- The postman brought me a large package. 邮递员给我送来一个大包裹。
- The package was tied with gay ribbons. 包裹用色彩鲜艳的丝带捆扎好。
- He carried the package in the crook of his arm. 他臂弯里挎着一个包裹。