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- Oridonin is the the main antitumor constituents of Donglingcao. 冬凌草甲素是中草药冬凌草抗肿瘤的主要活性成分。
- Abstract：ObjectiveTo determine the optimal extracting process of oridonin. 目的确定冬凌草中冬凌草甲素的最佳提取工艺。
- Content of oridonin in Rabdosia rubescens was 0.6152%, and the average recovery was 97.86% with RSD of 2.14%. 测得冬凌草叶中冬凌草甲素含量为0.;6152%25，平均回收率为97
- RESULTS Content of oridonin in Rabdosia rubescens was 0.6152%, and the average recovery was 97.86% with RSD of 2.14%. 结果测得冬凌草叶中冬凌草甲素含量为0.;6152%25，平均回收率为97
- RESULTS Content of oridonin in Rabdosia rubescens was 0.6152%,and the average recovery was 97.86% with RSD of 2.14%. 结果测得冬凌草叶中冬凌草甲素含量为0.;6152%25;平均回收率为97
- Abstract: OBJECTIVE To extract oridonin from Rabdosia rubescens and determine the content of oridonin. 文章摘要: 目的 从冬凌草中提取冬凌草甲素并测定其含量。
- After recrystallizing the oridonin content is determined by TIC Scanning to be as high as 99.28%. 经重结晶后用薄层扫描法测定含量达99.;28%25;是一种简便易行的方法。
- The oridonin contents can be controlled by control the amout of the leaves in Blushred Rabdosia. 建议通过控制药材的含叶量来控制冬凌草片中冬凌草甲素的含量。
- Methods: It was studied by orthogonal experimental design and oridonin in extraction was determined by HPLC. 方法：通过正交试验，以HPLC法测定提取物中冬凌草甲素的含量为指标进行研究。
- OBJECTIVE To extract oridonin from Rabdosia rubescens and determine the content of oridonin. 摘要目的冬凌草中提取冬凌草甲素并测定其含量。
- After recrystallizing the oridonin content is determined by TLC Scanning to be as high as 99.28%. 经重结晶后用薄层扫描法测定含量达99.;28%25;是一种简便易行的方法。
- METHODS Microwave technology was used to extract oridonin and the content of oridonin was determined by HPLC. 方法采用微波萃取技术提取,HPLC法测定。
- In the process of large-scale extracting research of oridonin, a large amount of components of pigment and fat were also extracted as impurities. 在进行冬凌草规模化提取研究中,提取冬凌草甲素的同时还会夹杂大量色素及油脂类杂质,严重干扰冬凌草甲素的分离纯化。
- Results:Oridonin could decrease the activity of telomerase, as well as cause apoptosis and inhibit the growth of K562 cells significantly. 并呈现出明显的量-效与时-效关系。
- We assume that the structural modification of oridonin would produce novel drug candidates with improved solubility and higher potency than oridonin. 本文试从药理作用、构效关系及其衍生物等方面对冬凌草甲素的研究概况作一综述。
- Objective: To investigate the change of Bcl-2 expression and telomerase during the apoptosis induced by oridonin on human hepatocelluar carcinoma BEL7402 cells. 目的:探讨冬凌草甲素诱导人肝癌细胞BEL-7402凋亡中Bc l-2表达及端粒酶活性的变化。
- Oridonin is an ent-kaurene diterpenoid compound mainly isolated from the leaves of R.rubescen.Oridonin has been proved to possess good antitumor activity. 冬凌草甲素是从冬凌草等植物叶子中提取出的一种贝壳杉烯二萜类化合物,具有好的抗肿瘤活性。
- By means of liqid scintillation count technique the effect of oridonin on the incorporation of [~3H] TdR, [~3H] UR and [~3H] leucine into the ECA cells in vitro was studied. 采用[~3H]TdR、[~3H]UR和[~3H]leucine在体外参入ECA细胞的液体闪烁计数术,探讨冬凌草甲素对DNA、RNA和蛋白质合成的影响。
- Methods:The variation of both morphology and inhibitory rate of K562 cells were observed in culture medium with various concentrations of Oridonin at different time in vitro. 方法:以不同浓度的冬凌草甲素作用于体外培养的K562细胞,检测细胞生长抑制率,观察细胞凋亡时的形态学变化,对细胞凋亡前后的端粒酶活性进行检测。
- The separation and prepartion methods of oridonin by medium pressure colunm chromatography have the advantages of fast speed,high efficiency,harmlessness,safety and economy. 采用中压柱层析法提取分离冬凌草甲素,具有洗脱速度快,分离效率高,溶剂无毒无污染,安全、价廉、易得等优点。
