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- normalized cDNA library 标准化cDNA文库
- Construction of cDNA library of peripheral blood leucocytes in normal Cyprinus carpio L. 正常鲤外周血白细胞cDNA文库的构建。
- An Improved Method for Solid-phase cDNA Library Construction. 一种改进的cDNA文库固相构建法。
- Construction of cDNA library from Sophora alopecuroides L. 苦豆子cDNA文库的构建。
- BAL cells from the patients with lung fibrosis expressed more mRNA species, whose copies existed in PMA stimulated U937 cell cDNA library, than those from normal volunteers. 肺纤维化患者的BAL细胞可表达出一些正常志愿者BAL细胞未表达的mRNA,而这些mRNA的拷贝存在于PMA刺激的U_(937)细胞cDNA文库中。
- Construction and Analysis of cDNA Library from the Leaf of Coleus blumei Benth. 彩叶草叶片cDNA文库的构建与分析。
- Methods Screening human placenta cDNA library with rat C/EBP cDNA probe. 方法以大鼠C/EBPcDNA为探针,筛选人胎盘cDNA文库。
- RNA was extracted from a white young alpaca and the full-length cDNA library was constructed by SMART technology. 从白色青年羊驼皮肤提取总RNA,并分离mRNA,利用SMART技术,构建了羊驼皮肤全长cDNA 文库。
- A high throughput in silico data analysis system for cDNA library, CLASP,was constructed by bioinformatic approaches. 应用生物信息学方法,构建了一套针对cDNA或EST文库的高通量、自动化分析体系,CLASP(cDNA Library Analysis SystemPrimary)。
- The cDNA library of the liver and kidney from the Vibrio anguillarum-infected Haliotis discus Hannai was constructed. 用鳗弧菌(Vibrio anguillarum)人工感染皱纹盘鲍(Haliotis discus Hannai),取等量肝和肾组织混匀,Unizol提取总RNA。
- A1200 and pRT! 05. The Suuccki cDNA library were driven by the cauliflower mosaic virus (CaMV) 35S promoter. 过量表达载体由pCAMBIA1200和pRT105组装而成,使得cDNA文库在CaMV35S启动子作用下过量表达。
- Full-length cDNA pool is the most important step in construction of cDNA library. 而全长cDNA池(full-length cDNA pool)则是cDNA文库构建的最重要环节。
- GAM molecule was taken out from human placenta cDNA library by using GST?gp130 fusion protein as a probe. 目的对以GST-gp130融合蛋白为探针,筛选人胎盘cDNA文库而得到的一个分子(GAM,gp130associatedmolecule)cDNA序列进行测定并做真核表达。
- The homologue of monkey ESc-615 was cloned in rat by screening rat epididymis cDNA library. 为得到该基因在大鼠中的同源基因,采用了筛选大鼠附睾cDNA文库的方法,得到了猕猴附睾ESc-615在大鼠附睾中的同源基因。
- The cDNA library of adult and newborn larvae of Trichinella spiralis was screened by a probe T54 from NBL subtracted cDNA library. 用旋毛虫新生幼虫差减cDNA文库中的T5 4克隆作为核酸探针 ,对旋毛虫成虫和新生幼虫混合cDNA文库进行筛选 ,获得 1个全长 14 6 4bp的cDNA分子。
- We used two fragments of SOX4, SOX4(aal-133)and SOX4(aa 130-380), as baits respectively, to screen human mammary cDNA library. 我们分别以人SOX4的两个片断SOX4(aa1-133)和SOX4(aa130-380)分别作为诱饵,从人乳腺文库中筛选与其相互作用的蛋白质。
- AIM: To construct a yeast two-hybrid cDNA library of hydroxycamptothecin-resistant human colon cancer cell line SW1116/HCPT in yeast cells. 目的:构建羟基喜树碱耐药结肠癌细胞株SW1116/HCPT细胞的酵母双杂交cDNA文库.
- In the yeast two-hybrid screens, we used the EFP and eif5a domain of eIF5A as baits to screen an oligo(dT)-primed human mammary cDNA library. 在酵母双杂交实验中,我们分别以eIF5A的两个结构域EFP(N末端)和eif5a(C末端)为诱饵,在人类乳腺文库中筛选与其相互作用的蛋白质。
- To construct subtractive cDNA library from the bone of apical facets in AIS patient with suppression subtractive hybridization (SSH) technique. 通过构建AIS患者脊柱关节突骨组织抑制消减cDNA文库,寻找AIS患者顶椎凸、凹侧关节突骨组织的差异表达基因,探讨AIS可能的病因。
- To screen PwA cDNA library with IRS firstly reported. Pw 2 and Pw 4 may apply to immunodiagnosis and immunoprophylaxis of paragonimiasis. 用卫氏肺吸虫成虫多抗筛选成虫 c DNA文库 ;所获 P.;w- 2和 P