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- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- multiplex RT PCR 多联RT-PCR
- Development and application of multiplex PCR assay for the detection of Campylobacter spp. 弯曲菌多重PCR检测方法的建立及其初步应用。
- Conclusion The pandemic group-specific multiplex PCR assay can fast identify pandemic vibrio parahaemolyticus and providing a methodology for searching epidemic pathogen. 结论群特异多重PCR法能快速鉴定当前副溶血性弧菌流行群,有利于流行病学溯源等。
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus,develope a pandemic group-specific multiplex PCR assay for base unit. 目的建立适于基层单位应用的针对副溶血性弧菌流行群的特异多重PCR方法。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus, develope a pandemic group-specific multiplex PCR assay for base unit. 摘要目的建立适于基层单位应用的针对副溶血性弧菌流行群的特异多重PCR方法。
- Reverse transcription polymerase chain reaction (RT PCR) assay was used to detect the expression of MDR 1 mRNA and MRP mRNA in 35 patients. Meantime, Immunohistochemistry SABC technique was used to detect P glycoprotein(P gp) in 61 cases. 应用逆转录-多聚酶链式反应(RT-PCR)技术,检测了35 例喉癌患者MDR-1 m RNA、MRPm RNA 基因的表达,同时,应用免疫组化SABC法检测了61 例患者MDR-1 基因产物P-gp。
- The recombination plasmid was transiently transfected into COS7 cells and the expression of the fusion protein was identified by RT PCR, immunofluorescent assay and sandwich ELISA. 以其瞬时转染COS7细胞 ,应用RT PCR、细胞免疫荧光技术 ,及夹心ELISA检测融合蛋白的表达。
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- Objective To establish a PCR assay for the detection of Helicobacter pylori. 目的建立一种PCR方法检测幽门螺杆菌(Helicobacter pylori,Hp)感染。
- Objective To establish PCR assay of Porcine Parvovirus detection in Trypsin. 目的建立原材料胰蛋白酶中猪细小病毒的PCR检测方法。
- RT PCR for EWS FLI 1 is sensitive enough to detect minimal residual Ewing's sarcoma cells in the bone marrow. 以EWS FLI 1融合基因为标志的反向PCR测定骨髓Ewing肉瘤细胞的灵敏度为 10 -6,它是测定骨髓Ewing肉瘤细胞高度敏感的方法 ;
- The positive rate of MDR1 mRNA was 70.3% with improved RT PCR in 37 among 49 cases of ovarian carcinomas. 有37 例卵巢癌用改进的RT?PCR 检测MDR1 基因m RNA 表达的阳性率为70?3 %25 。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT-PCR技术从人B淋巴瘤细胞系Raji中克隆到B7-1(CD80)cDNA,并经测序证实。
- Methods B7 expresion was studied by means of RT PCR and immunohistochemistry (IH) in 45 tumors of the urinary system. 方法应用逆转录?聚合酶链反应(RT?PCR)和免疫组织化学方法,检测45例泌尿系肿瘤B7基因在mRNA和蛋白水平的表达情况。
- Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR). 方法采用逆转录聚合酶链反应(RT-PCR)检测84例静脉毒瘾者血浆标本。