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- Methods The full length of CD95 cDNA was inserted into the multiple cloning site of the expression vector pBK CMB by molecular cloning technique. 方法 采用分子克隆技术将CD95基因插入真核表达载体pBK CMV的多克隆克隆位点之间 ,以脂质体介导法将目的基因导入受体细胞SGC790 1,用G418筛选克隆细胞 ;
- METHODS: The HAb18GEF gene fragment was amplified by PCR and digested with Sac I/Not I,and then cloned into the multiple clone site downstream of EpoR/LR-F3 gene of eukaryotic expression vector pCEL2f. 方法:用PCR法扩增肝癌相关抗原HAb18G胞外段基因,SacI/NotI双酶切后插入真核表达载体pCEL2f中,构建EpoR/LR-F3/HAb18GEF嵌合受体基因的重组表达载体pCEL2f/HAb18GEF,并经酶切和测序验证。
- The products of PCR werenamed Sa and Sb, of 2.1Kb and 2.3Kb respectively. Sa was inserted in EcoR I andKpn I sites after Sb was cloned in KpnI and PstI multiple cloning sites of the samepUC18 plasmid. 1Kb和2.;3Kb两个片段，经酶切鉴定后，先后插入到pUC18质粒载体上的EcoRI和PstI多克隆位点上，构建了重组质粒pUC-S。
- The gene segments mentioned above were cloned into the multiple cloning sites of retrovirus vector pLXSN to construct an attenuated superantigen expression vector pLXSN SEA (D227A) linker CD80tm modulated by cis acting element of AFP gene. 将上述片断插入逆转录病毒载体 pLXSN的多克隆位点 ,构建AFP基因顺式作用元件调控的肝癌特异性减毒超抗原表达载体 (pLXSNSEA(D2 2 7A) linker CD80tm)。
- multiple clone site 多克隆位点
- multiple cloning site 多克隆位点
- multiple cloning sites 多克隆位点(分)
- The first item: monoclone antibody and multiple clone antibody have exerted abroad in biology research, clinic diagnosisand therapy field.It"s a huge market. 其中即将上马的第一个项目：单克隆抗体和多克隆抗体在生物学研究、临床诊断和治疗等领域中已被广泛运用，具有非常广阔的市场前景。
- In the end, an expression T vector pBV-HX, which is the first practical use of the HphI-XcmI linker as a universal cloning site, was constructed by using the linker insert into vector pBV220. 将HphI-XcmI接头接入pBV220中构成一表达T载体是该接头的第一个实际应用。
- While most of Baker's clone characters are recorded separately, he sometimes voices multiple clones at once, depending on the complexity of the dialogue and the number of clones in the scene. 虽然绝大多数贝克的克隆人角色都是分别录音的，但他有时也要根据对话的复杂程度以及场景中的克隆人数，一次配多个克隆人。
- Methods: A cDNA fragment from the mRNAs of skeletal muscle of 13 day old chicken embryo was amplified by RT PCR method, and inserted into pGEM and pUC vectors at clone sites and sequenced respectively. 方法 :从 13d鸡胚骨骼肌mRNAs经RT PCR方法扩增RTEF 1的编码序列 (cDNA) ,将其克隆到 pGEM和 pUC载体并进行核苷酸序列分析。
- He is the person with multiple injuries. 他就是那个多处受伤的人。
- Friendship multiple joy and divide grieve. 朋友之间，欢乐同享，苦难同当。
- We use the hall for multiple purposes. 这个大厅我们派多种用途。
- A multiple crash can often be seen on that motorway. 那条公路上的联环撞车事故很多。
- So who is most likely to want or use human cloning? 所以谁最有可能需要或使用人类克隆技术呢?
- Expression and purification of enhanced green fluorescent protein and preparation of multiple cloning antibody 增强型绿色荧光蛋白原核表达纯化与多克隆抗体的制备
- Cloning differs from genetic engineering. 克隆与基因工程不同。
- These are inserted into a cloning vector. 它们被插入克隆载体。
- The actual process of cloning is very technical. 克隆的实际过程有很强的技术性。
- 今日热词
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries
