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- The separation of primary mouse embryonic fibroblast (PMEF) and human embryonic fibroblast (HEF). 小鼠胚胎成纤维细胞(PMEF)和人胚胎成纤维细胞(HEF)的分离培养
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词：胚胎成纤维细胞；
- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- RESULTS: Under the optical microscope, mouse embryonic fibroblasts were irregular, rapidly grew, passaged at 1:4.Following mitomycin C treatment, cells slowly adhered and could not proliferate. 结果：光镜下小鼠胚胎成纤维细胞呈不规则形，生长较快，传代比例为1:4，经丝裂霉素C处理后细胞贴壁较慢，失去分裂增殖能力。
- mouse embryonic fibroblasts (MEFs) 小鼠胚胎成纤维细胞
- Preliminary proteome analysis of mouse embryonic fibroblast conditioned medium 小鼠胚胎成纤维细胞条件培养液的蛋白质组学初步分析
- Culture of ICR mouse embryonic fibroblasts and production of feeder layers for embryonic stem cells ICR小鼠胚胎成纤维细胞的培养及饲养层的制作
- Effect of mouse embryonic fibroblast feeder layer and liver matrix of rabbit on the activities of LDH and ACP in cultured cells from schistosomula Schistosoma japonicum 鼠胚胎成纤维细胞饲养层及其与肝基质联合对日本血吸虫童虫培养细胞LDH、ACP影响的研究
- Effects of mouse embryonic fibroblast feeder layers and combined of mouse embryonic fibroblast and collagen of rat tail on adhesion of cultured cells from Schistosoma japonicum 鼠胚胎成纤维细胞饲养层及与鼠尾胶联合促日本血吸虫培养细胞贴壁作用的研究
- 90 and 96 hours embryos were plated on ME(mouse embryonic fibroblast cells)feeder layer, using DMEM/F12 medium with 10% FBS and 10%rabbit serum supplemented with LIF or EGF. 建系条件为，使用小鼠原始胚胎成纤维细胞（ME）作饲养层，以含10%25胎牛血清和10%25兔血清的DMEM/F12为培养基，添加白血病抑制因子（LIF）或上皮生长因子（EGF），胚龄为90，96h。
- mouse embryonic fibroblasts 小鼠胚胎成纤维细胞
- mouse embryonic fibroblast 胎鼠成纤维细胞
- mice embryonic fibroblasts 鼠胚成纤维细胞
- A characteristic cytopathic effect of CMV presented in a culture of human embryonic fibroblasts with conjunctival swab. 这些有病变反应之细胞经免疫萤光染色检定，对巨细胞病毒单株抗体染色呈阳性反应。
- Feeder cells with murine embryonic fibroblasts(MEF) were better than that with human embryonic fibroblasts(hEF), or STO or bovine embryonic fibroblasts (bEF). MEF、STO、bEF和hEF可提高人原始生殖细胞(PGCs)的贴壁率,能促进PGCs源的人类胚胎干细胞(ES)的分离效率; 以MEF作为饲养层效果较好。
- Methods Anti-NOX4 ribozyme clones of mouse embryonic stem (ES) cells were differentiated into embryoid bodies (EBs). 方法用核糖酶技术获得尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4低表达小鼠胚胎干细胞克隆,将小鼠胚胎干细胞分化为胚小体。
- Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization. 方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
- Objective To investigate the preparation methods of feeder cells of mouse embryonic stem cell. 摘要目的：探讨小鼠胚胎干细胞饲养层细胞的制备。
- mouse embryonal fibroblast cell 小鼠胚胎成纤维细胞
- Objective: To establish an effective purification method for primary culture of mouse embryonic palatal mesenchymal(EPM) cells. 目的:建立纯化原代培养的小鼠胚胎腭突间充质细胞的纯化方法。
