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- The separation of primary mouse embryonic fibroblast (PMEF) and human embryonic fibroblast (HEF). 小鼠胚胎成纤维细胞(PMEF)和人胚胎成纤维细胞(HEF)的分离培养
- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词:胚胎成纤维细胞;
- Preliminary proteome analysis of mouse embryonic fibroblast conditioned medium 小鼠胚胎成纤维细胞条件培养液的蛋白质组学初步分析
- RESULTS: Under the optical microscope, mouse embryonic fibroblasts were irregular, rapidly grew, passaged at 1:4.Following mitomycin C treatment, cells slowly adhered and could not proliferate. 结果:光镜下小鼠胚胎成纤维细胞呈不规则形,生长较快,传代比例为1:4,经丝裂霉素C处理后细胞贴壁较慢,失去分裂增殖能力。
- Effect of mouse embryonic fibroblast feeder layer and liver matrix of rabbit on the activities of LDH and ACP in cultured cells from schistosomula Schistosoma japonicum 鼠胚胎成纤维细胞饲养层及其与肝基质联合对日本血吸虫童虫培养细胞LDH、ACP影响的研究
- Effects of mouse embryonic fibroblast feeder layers and combined of mouse embryonic fibroblast and collagen of rat tail on adhesion of cultured cells from Schistosoma japonicum 鼠胚胎成纤维细胞饲养层及与鼠尾胶联合促日本血吸虫培养细胞贴壁作用的研究
- mouse embryonic fibroblasts (MEFs) 小鼠胚胎成纤维细胞
- 90 and 96 hours embryos were plated on ME(mouse embryonic fibroblast cells)feeder layer, using DMEM/F12 medium with 10% FBS and 10%rabbit serum supplemented with LIF or EGF. 建系条件为,使用小鼠原始胚胎成纤维细胞(ME)作饲养层,以含10%25胎牛血清和10%25兔血清的DMEM/F12为培养基,添加白血病抑制因子(LIF)或上皮生长因子(EGF),胚龄为90,96h。
- mouse embryonic fibroblast 胎鼠成纤维细胞
- Culture of ICR mouse embryonic fibroblasts and production of feeder layers for embryonic stem cells ICR小鼠胚胎成纤维细胞的培养及饲养层的制作
- Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization. 方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
- mouse embryonal fibroblast cell 小鼠胚胎成纤维细胞
- mouse embryonic fibroblasts 小鼠胚胎成纤维细胞
- Methods Anti-NOX4 ribozyme clones of mouse embryonic stem (ES) cells were differentiated into embryoid bodies (EBs). 方法用核糖酶技术获得尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4低表达小鼠胚胎干细胞克隆,将小鼠胚胎干细胞分化为胚小体。
- Objective To investigate the preparation methods of feeder cells of mouse embryonic stem cell. 摘要目的:探讨小鼠胚胎干细胞饲养层细胞的制备。
- The virus was continually inoculated 5 generations onto rabbit kidney cell and chicken embryonic fibroblast cell (CEF) and induced typical cytopathic effect (CPE). The typical CPE cloud be seen when culturing the virus on RK-13 cell . PK-15cell . 该分离毒株在兔肾原代细胞上(RK)上和鸡胚成纤维细胞(CEF)上连传5代均出现典型细胞病变,再接种RK-13细胞、BHK-21细胞、Vero细胞、PK-15细胞和143 TK~-细胞均出现典型细胞病变,在RK-13细胞上的感染滴度为10~(6.;52)TCID_(50)/0
- Objective: To establish an effective purification method for primary culture of mouse embryonic palatal mesenchymal(EPM) cells. 目的:建立纯化原代培养的小鼠胚胎腭突间充质细胞的纯化方法。
- "Dicer-Deficient Mouse Embryonic Stem Cells Are Defective in Differentiation and Centromeric Silencing." Genes &Development 19, no. 4 (2005): 489-501. 第二篇用遗传学诠释了非编码在哺乳动物中心体沉默中的重要作用。
- Objective To examine the effect of human oviductal epithelial cells on early mouse embryonic cleavage and growth in vitro. 目的了解2细胞期小鼠胚胎与人输卵管上皮细胞体外无血清共培养中的发育状况。