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- Objective To choose the metal ions of immobilized metal affinity chromatography for separating the inclusion body of recombinant hepatitic E virus. 目的选择固相金属亲和层析柱的金属离子,以优化分离纯化重组戊型肝炎病毒包涵体的条件。
- Methods: The phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography(PMAC) resin. 方法:利用金属磷酸盐亲和层析树脂富集人肾组织磷酸化蛋白。
- Methods: The phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography (PMAC) resin. 方法：利用金属磷酸盐亲和层析树脂富集人肾组织磷酸化蛋白。
- Abstract： Immobilized metal affinity chromatography (IMAC) is a well-established protein separation technique experiencing expanded use based on new, high-performance analytical phases. 摘要：固定化金属离子亲和色谱（IMAC）作为一种新型高效色谱分离模式，已经被广泛应用于蛋白质的分离分析。
- immobilized metal affinity chromatography (IMAC) 固相金属亲和层析
- immobilized metal affinity chromatography(IMAC) 固定化金属亲和层析
- Preparation and Chromatographic Behavior of Continuous Rod Column for Immobilized Metal Affinity Chromatography 一种金属螯合连续棒色谱柱的制备及色谱性能
- Keywords pemphigus;desmoglein;chimeric toxin molecule;metal affinity chromatography; 关键词天疱疮;嵌合毒素;原核表达;金属鏊合亲和层析;
- Keywords immobilized metal affinity chromatography（IMAC）;recombinant human-like collagen;purification; 固相金属亲和层析;重组类人胶原蛋白;纯化;
- Resolution and Purification of Recombinant Human Manganese Superoxide Dismutase by Immobilised Metal Affinity Chromatography 金属螯合亲和层析分离纯化重组人锰超氧化物歧化酶
- metal affinity chromatography 金属亲和层析
- The affinity chromatography can be used in the purification of Fab. 我们可以经过亲和层析 ,纯化基因工程抗体Fab片段
- The expressed product issoluble, MW 18kD, which was purified by Ni+ metal chelate affinity chromatography. 表达产物为可溶性蛋白，分子量约为18kD，通过Ni~+离子亲和层析一次性纯化。
- Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate. 制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography(MCAC). 研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
- The purified 6A5 from ascites was about 1.1 mg per ml ascites with affinity chromatography method. 经免疫亲和层析法纯化;腹水中抗体蛋白的得率为1.;1 mg/ml。
- Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表达产物为包涵体形式，溶解和折叠后，以GST亲合层析色谱纯化。
- Made sheep anti?human IgG Fab Sepharose 4B affinity chromatography, purified the souble Fab fragment. 制备羊抗人IgGFab抗血清、建立抗人IgGFab抗体－Sepharose4B亲和层析柱，纯化Fab片段。
- The supernant flows through Ni-NTA gel for affinity chromatography, and thepurity is over 91.5%. 上清经Ni-NTA凝胶亲和层析;纯度达到91.;5%25以上。
