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- A mammalian expression vector(pSV_2,-EP) was reconstructed by inserting a oligonucleolide fragment into pSV_2dhfr plasmid. 对pSV_2dhfr质粒进行改造,切除dhfr基因,加入一个人工合成的寡聚核苷酸片段,构建成哺乳动物细胞表达载体(pSV_2-EP)。
- Objective:To construct the recombinant HBV mammalian expression vectors by insertion of CpG motif and IL-2 gene respectively. 目的 :构建插入CpG序列和IL - 2基因的HBV重组真核表达载体。 方法 :采用PCR产物直接克隆方法 ;构建了编码HBsAg基因的真核细胞表达载体pcDNA3.;1+ -HBsAg ;
- The construction and the evaluation of expression efficiency of an inducible mammalian expression vector 可诱导型真核表达载体的构建及其表达效率的测定
- CONSTRUCTION AND EXPRESSION OF BASIC FIBROBLAST GROWTH FACTOR MAMMALIAN EXPRESSION VECTOR 碱性成纤维细胞生长因子真核表达载体的构建及表达
- In this report, the coding region of human angiopoietin-2 gene (Ang-2), a 1491 bp fragment, was cloned into mammalian expression vector pcDNA3; 本实验获得Ang-2的完整编码序列,经过核苷酸序列分析,成功的构建了含有1491bp的人Ang-2基因真核表达载体;
- Objective To construct a eukaryotic expression vector for expression of human neuropathy target esterase(NTE) esterase domain in mammalian cells. 目的构建人神经病变靶标酯酶(NTE)酯酶活力域(NESP)真核表达载体,并在细胞中表达。
- CONSTRUCTION OF RECOMBINANT HBV MAMMALIAN EXPRESSION VECTORS BY INSERTION OF CpG MOTIF AND IL-2 GENE RESPECTIVELY 插入CpG序列和IL-2基因的HBV重组真核表达载体的构建
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题: 图1.;穿梭表达载体pRL_hEGF的构建。
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- But the low level of mammalian expression and laggard fermentation process constrained the development of antibody industry in China. 但是目前国内动物细胞表达水平普遍较低和发酵工艺落后的现状制约了我国抗体药物产业化的发展。
- To construct mammal expression plasmid pcDNA 3.1 ( + )/GDF-5 and check the expression of it in bone marrow mesenchyal stem cells of mice. 目的:通过基因重组技术体外构建真核表达质粒pcDNA3.;1(+)/GDF-5;并检测其在小鼠骨髓基质干细胞中的表达。
- CBF1 expressing stable Kusa-A1/CBF1 cell line was generated by the Flp-In mammalian expression system. 方法采用基因转染法建立CBF1稳定过表达细胞系Kusa-A1/CBF1,检测其成骨活性。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9,将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接获得250bp的目的基因序列,将目的基因克隆于pGAP9K,获得组成型表达载体pGAP9K-gat。