您要查找的是不是:
- Optimization on the condition of liposome mediated transfection to COS 7 cell line 用脂质体法转染 COS-7 细胞条件的优化
- METHODS: The eukaryotic expression vector pcDNA3.0 containing human full length HAb18G cDNA and empty vector pcDNA3.0 were introduced respectively into NIH/3T3 fibroblast cells by liposome mediated gene transfection method. 方法 :将含有肝癌相关抗原HAb1 8G全长cDNA的真核表达载体 pcDNA3.;0 /HAb1 8G利用脂质体介导法转染小鼠成纤维细胞NIH/ 3T3;G4 1 8筛选建立稳定表达阳性克隆细胞株t3T3; 利用流式细胞仪、间接免疫荧光染色方法鉴定目的蛋白转染情况 ;
- Con-elusion:The suicide gene therapy system using an ovarian-specific promoter by polyethylenimine mediated transfection has a targeting killing activity on human ovarian cancer. 结论:多聚乙烯基亚胺介导的卵巢特异性启动子调控下的自杀基因对卵巢癌有靶向杀伤作用。
- Cationic liposome mediated NT-3 gene to transfect mice spiral ganglion cells 阳离子脂质体介导小鼠原代螺旋神经节细胞NT-3基因转染的实验研究
- Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. 方法 将外源性neo基因转染人胚胎成纤维细胞 ,作为饲养层细胞用于ES细胞转基因后筛选及增殖过程的培养。
- AIM:To investigate the expression of cationic liposome mediated neurotrophin 3(NT 3) gene in injured spinal cord of rats,and observe the effect of exogeno us NT 3 on the regeneration of injured axons. 目的:研究阳离子脂质体介导的神经营养因子3基因在大鼠损伤脊髓内的表达,观察外源性神经营养因子3对损伤脊髓轴突再生的作用。
- Experimental study of transfection of cochlea by SA liposome mediated reporter gene SA脂质体介导报告基因转染耳蜗的实验研究
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
- Experiment of Liposome Mediated Glial Growth Factor Gene Transfer in Vivo on Recovery of Spinal Cord Injury in Rats 脂质体介导胶质细胞生长因子基因体内转染对大鼠脊髓损伤修复作用的实验研究
- liposome mediated gene transfer 脂质体介导基因转移
- Cellular transfection and localization observation were performed to eucaryotic cells under mediation with liposome. 脂质体介导真核细胞进行转染并细胞定位观察。
- This structure is termed a liposome. 这种结构被称为微脂粒。
- Objective To explore the effect of antisense c-myc on human glioma cell U251 after transfection by dint of non-phospholipid liposome (NPL). 目的 确定非磷脂脂质体 (non phospholipidliposome ,NPL)介导反义c myc基因转染人脑胶质瘤 (glioma)的作用。
- Objective:To prepare oxaprozin liposome. 目的:制备恶丙嗪脂质体。
- Antibody mediated phagocytosis by macrophages. 抗体介导的巨噬细胞吞噬的测定。
- He mediated in the quarrel between the two boys. 他调解两个孩子之间的争吵。
- Then it was introduced into COS7 cells by liposome transfection and it's expression was examined by Western blot and immunolocalization. 脂质体法转染COS7细胞后,运用Western印迹及免疫定位技术检测表达产物。
- AIM To investigate the factors affecting transfection efficiency of antisense oligodeoxynucleotides (ASON) by cationic fusogenic liposome (CFL). 目的研究阳离子膜融合脂质体(CFL)介导反义寡核苷酸(ASON)的细胞转染效率及影响因素。
- And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection. 结果经测序分析;各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞;48h后可观察到细胞表面4-1BB分子明显下调;其胞内mRNA水平也显著降低;与无关干扰组比较;差异有统计学意义(P<0.;05)。
- Father mediated in the quarrel between the two girls. 父亲调解两个女孩之间的争吵。