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- lipofectamin transfection 脂质体法
- Human brest cancer cell MCF-7 was transfected with pcDNA3.1(-)/VEGF-C by using lipofectamine transfection reagent and screened with antibiotic G418. The expression of VEGF-C protein in CHO cell and supernatant was identified with Western-blotting. 最后用Western -blotting法检测细胞培养上清以及裂解细胞中的VEGF -C蛋白。
- After ATF gene transfection, the ATF mRNA was obviously expressed. 结果表明,转染ATF基因后,肿瘤细胞呈现ATF基因的明显表达;
- Transfection, make stable C/EBP p expression cell line. 质粒转染，制备稳定表达C尼BPp的细胞系。
- Cell density was associated with PEI/DNA transfection efficiency. 细胞密度影响转染效率;
- Gene transfection is the key technique of gene therapy. 摘要基因治疗的关键技术是基因转移。
- Objective To construct Giardia canis virus(GCV) transfection vector. 目的构建犬贾第虫病毒(Giardia canis virus,GCV)转染载体。
- GFP can be used as a reporter system for optimizing transfection of K562 cells. 结论：电转染较脂质体转染K562细胞的效率高，GFP可作为报告系统优化K562细胞转染条件。
- The inhibitory effect of gene transfection on solid tumor was observed in mice. 小鼠实体瘤块模型研究基因转染抑制肝癌生长作用。
- Hoechst and HE staining showed that apoptosis was induced after transfection. 细胞HE染色部分细胞呈典型的凋亡细胞形态学改变；
- Positive Ion Lipoplexes and Its Application in Gene Transfection and Gene Therapy. 阳离子脂质体及其在基因转移和基因治疗中的应用。
- The study of gene transfection into rabbit's tongue muscle in vivo and optimization. 分子外科缝线术的建立及其优化方案的研究。
- Methods The pAdIL-10 constructed by our laboratory was transferred into HEK 293 cells by lipofectamin for packing, and the recombinant replication-deficient adenovirus, AdIL-10, was finally obtained. 方法 将本实验室自行构建的重组腺病毒质粒pAdIL-10用Lipofectamin包装转染HEK 293细胞，即可获得重组腺病毒AdIL-10。
- RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression. RT-PCR结果显示:E1A基因在Hela细胞中能稳定表达,E1A基因的转染明显降低了Her-2基因在Hela细胞中的表达水平;
- Objective To optimize the transfection rate in COS 7 cell line with electroporation method. 目的 以电穿孔法转染COS 7细胞系以得到较高的转染率。
- Objective To study the effect of ULBP3 gene transfection on the sensitivity of Saos-2 cell line to human NK killing. 目的 观察ULBP3基因转染对人外周血NK细胞对Saos -2细胞杀伤活性的影响。
- Exogenous hTERT gene transfection could promote the proliferative capacity of hEF. 外源性hTERT基因转染可使hEF增殖能力增强。
- This article reviews about progress in gene transfection technique to myocardium i... 本文仅就目前体内心肌基因转染技术的进展作一综述。
- Methods:The plasmid pcDNA3 containing the wild type p16 gene and neo gene was used in the transfection experiment. 方法：采用pcDNA3质粒进行转染，该质粒含有野生型P16基因片段和新霉素抗性基因。
- Transfection showed an efficiency of 90% in ADAS cells. BMP-7 expression in ADAS cells was identified by Western blot. 早期对ADAS cells的转染效率可达90%25, Western-Blot方法检测到第1周与第8周的转染组细胞均有蛋白水平的表达。
