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- The stable isotope labeling technique provided an ideal method for accurately quantitating the low abundance proteins which play a key role in cells or tissues. 近几年来,定量蛋白质组的技术发展很快,稳定同位素标记技术的提出,为准确定量在细胞或组织体系中发挥重要功能的低丰度蛋白质提供了一个理想的方法。
- isotope labelling technique 放射性核素标记技术
- isotope labelling techniques 同位素标记技术
- isotope labeling technique 核素标记技术
- The basic principle,procedure and examples of the dansyl chloride fluorescence labelling technique are described. 介绍了用丹磺酰氯荧光标记法检测表皮角质层更新时间的基本原理、方法步骤和应用实例。
- Methods GABA receptor counts of peripheral branch of PTN (10 pairs painful infrior alveolar neuralgia and painless Lingual never) were measured by radioimmuoassay and isotope Labelling method. 方法通过同位素标记放射免疫法测定10对疼痛的下牙槽神经与无痛的舌神经末梢支GABA受体含量。
- Immunogold labelling technique was used for in situ ultrastructural detection of antibodes 51KD human sperm membrane antigen. 本文应用免疫金标记法,对抗51KD精子膜抗原的抗精子抗体在精子的定位进行了超微结构观察。
- The effect of lactic acid(AHA) on epidermal renewal was studied by the dansyl chloride labelling technique in the paper. 用丹磺酰氯标记技术研究了果酸对表皮更新的影响。
- Conclusion: Pre-embedding cationic colloidal gold (CCG) labelling technique can reveal the anionic sites on the surface of dural vascular endothelial cells qua ntitatively. 前包埋阳离子胶体金标记技术可特异性地显示硬脑膜血管内皮细胞表面的阴离子场,并可做定量研究。
- The application of Holo catheter labelling technique with anatomic imaging localization to achrieve the double way conduction block by radiofrequency catheter ablation of TVC TA isthmus, is a reliable method for treating atrial flutter. 应用Halo导管标测技术 ,采用解剖学影像定位法消融IVC TA峡部 ,以确定峡部双向传导阻滞 ,是射频消融心房扑动的可靠方法。
- In this paper, these novel protein isotope labeling methods and alternative protein expression systems are reviewed. 本文旨在对这些蛋白质标记表达新技术的方法及应用予以逐一介绍。
- Methods Model of vasoligation and vasostomy in male rat was established, and then ter- minal deoxynucleotidyl transferase-mediated dUTP nick labelling technique to detect the apoptosis of spermato- genic cells at 4, 8, 12, 16 weeks after vasostomy. 方法 以雄性 SD大鼠为研究对象 ,建立输精管阻断及阻断后复通模型 ,采用 DNA缺口原位标记凋亡细胞方法 ,观察大鼠生精细胞在输精管复通后 4、8、12及 16周的凋亡状况。
- Methods: The morphology of HSF was observed under an electron microscope. The cell cycle was analyzed with flow cytometry. 3H TdR uptake was tested with isotope labeled method. 方法电镜观察细胞形态,流式细胞仪测定细胞周期,同位素放射测定3H胸腺嘧啶核苷(3HTdR)摄取量。
- The BBB score and Rivlin plate, SEP, retrograde and label technique of Horseradish Peroxidase, gross observation, histological and morphological analyses were taken as the observed indices. 运用脊髓神经功能(BBB评分和Rivlin斜板法)、体感诱发电位、辣根过氧化酶(HRP)示踪、大体观察、组织学及形态计量分析、超微结构变化作为观察指标进行实验观察。
- Methods The proliferation assay,NK cell killing activity assay and immunologic fluorescence double label technique were used to study four sear effect on T cell immunologic function. 方法 经增殖试验、NK杀伤活性试验及免疫荧光双标记方法研究了 4种血清对T细胞免疫功能的影响。
- To study the use of nonradioactive isotope labeled substracts ~15N-Gly and ~13C-Palmitic Acid as tracers in measuring the adaptive absorptive function in a child having undergone total small bowel resection. 研究应用稳定同位素~(15)N-甘氨酸和~(13)C-棕榈酸示踪测定全小肠切除术后患儿的肠吸收功能。
- The metabolic kinetics of branched chain amino acids (BCAA) and aromatic amino acids (AAA) in normal subjects and patients with severe viral hepatitis as studied with 4 stable isotope labeled tracers. 作者用4种稳定性核素标记的支链氨基酸和芳香氨基酸对正常人与重型病毒性肝炎(重型肝炎)患者进行示踪动力学的研究。
- Colloid gold labelling technique 胶体金标记
- non-invasive labelling technique 非损伤性标记技术
- fluorimetric labelling technique 荧光计量法标记技术
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries