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- The result of enzyme activity showed that TSV PA protein expressed in insect cells had a higher activity. 酶活力测定结果表明 ,昆虫细胞表达的TSV PA蛋白具有较高活性
- Cyclin D1 and CDK4 proteins were expressed correctly in insect cells and had their biological activity. 成功构建了细胞周期蛋白D1及CDK4真核杆状病毒表达载体 ;并且在昆虫细胞中正确表达了具有生物活性的细胞周期蛋白D1及CDK4融合蛋白 .
- In the adherent culture, the virus did not result in cell lysis after the insect cells were infected. 而在本论文中，我们尝试将此一非溶裂昆虫细胞/杆状病毒表现系统转移到旋转瓶的悬浮培养。
- The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- Abstract: The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要: 昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- Objective Bac-to-Bac baculovirus expression system was used to obtain biologically active recombinant human basic fibroblast growth factor(hbFGF) in insect cells. 目的利用Bac-to-Bac杆状病毒表达系统,将人碱性成纤维细胞生长因子(human basic fibroblast growth factor,hbFGF)基因进行表达,并检测重组蛋白的特异性及生物学活性。
- Objective: The aim was to construct the recombinant of neuro related cell adhesion molecule(NrCAM) and immunoglobin Fc fragment and transfect it into insect cells. 目的 :构建神经原相关的细胞粘附分子 (NrCAM )和免疫球蛋白Fc片段融合基因的重组质粒并通过Baculovirus载体转染到昆虫细胞。
- Western blot analysis using this antiserum showed that the specific reaction to GP41 protein expressed in both prokaryotic cells with His fusion and viral infected insect cells. 该抗血清可与原核表达的His-GP41融合蛋白及在感染的昆虫细胞中表达的GP41蛋白发生特异性免疫反应。
- Then rAcV-Bac-bFGF was infected Sf21 insect cells again to express the hbFGF gene, its expression product was measured by SDS-PAGE and Western blotting. 进一步将重组病毒感染Sf21细胞,在Sf21细胞中进行表达,对表达产物进行SDS-PAGE电泳、Western bloting分析;
- In this paper, a MTT-based semiautomated colormetric assay was used to study the sensitivity difference among eight cultured insect cell lines to Methomyl pesticide. 采用MTT法，研究了灭多威农药对八种昆虫离体细胞系敏感性的差异。
- These indicated that pieces of E. coli and pieces of insect cell that expressed 16L1 proteins could replace purified HPV16L1 proteins as antigen in ELISA test. 表明用表达16L1的大肠埃希菌破碎物和表达16L1的昆虫细胞破碎物可以代替纯化的HPV16L1蛋白作为抗原进行ELISA试验。
- Summers M D,Smith G E.A manual of methods for baculovirus vectors and insect cell culture procedures[J].Texas Agricultural Experiment Station Bulletin,1987,1555-1556. 洪华珠彭建新.;一株高水平表达重组蛋白昆虫细胞系的建立[J]
- Co-transfection of SF9 insect cells was performed with rpVLgB and Baculovirus linear DNA (BAC-N-Blue DNA). After three times purification, a recombinant baculovirus, designated as rpVL-ILTVgB was obtained. 将rpVLgB转移载体质粒与杆状病毒DNA(Bac-N-Blue DNA)共转Sf9昆虫细胞,经三轮蚀斑纯化,获得的重组病毒命名为rpVL-ILTVgB。
- Insect cell lines have become useful tools with a variety of applications in such research fields as insect pathology, parasitology, endocrinology, genetics, molecular biology, etc. 昆虫细胞系在昆虫病理学、寄生虫学、内分泌学、遗传学和分子生物学等基础和应用研究中得到越来越广泛的应用。
- Most insect do not live in polar region. 大多数的昆虫不在极地地区生存。
- The insect bit him and drew blood from his arm. 那只昆虫叮在他的手臂上吸血。
- She catch the insect by invert her cup over it. 她把杯子倒扣在昆虫上，将它逮住了。
- Spodopterafrugiperda (Sfg) insect cells 草地夜蛾细胞
- She stamped on the insect and killed it. 她踩死了一只虫子。
- Human tissue is made up of cells. 人体的组织是由细胞构成的。
