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- Methods: The improved CTAB DNA extraction method. 方法:改良CTAB法。
- And the DNA concentration is higher than improved CTAB method. 而CTAB法抽提银杏胚乳DNA纯度较高,含杂质少。
- Method Genomic DNA was extracted by improved CTAB method. 方法采用改良CTAB法从罂粟叶片中提取DNA;
- In addition, the key steps and main features of the improved CTAB method are discussed. 并对改良CTAB法的关键步骤作了具体分析讨论。
- The DNA from mature leave extracted only by improved CTAB method could be suitable for RAPD analysis. 成熟叶用改良CTAB法提取也可得到适于RAPD分析的DNA;
- A Rapid Improved CTAB Method for Macro-extraction Genomic DNA in the Larvae of Diamondback Moth, Plutella xylostella L. 改进CTAB法快速大量提取小菜蛾幼虫基因组DNA
- Conclusion The improved CTAB technique can be used for extracting DNA from herbs riching polysaccharide. 结论改良CTAB法适用于类似玉竹这类含多糖较多的根及根茎类中药材DNA的提取。
- DNA A Rapid Improved CTAB Method for Macro-extraction Genomic DNA in the Larvae of Diamondback Moth, Plutella xylostella L. 改进CTAB法快速大量提取小菜蛾幼虫基因组。
- Improved CTAB method was used to extract the six tissues DNA, (such as sexual gland, zool, mantle, adductor muscle, gill, liver). 采用改良CTAB法提取蚌的性腺、斧足、外套膜、闭壳肌、鳃、肝脏六个主要组织器官的基因组DNA,发现性腺的提取效果最好,提取过程中研磨容易,水浴消化时间短,DNA电泳带型最清晰;
- The following results were obtained:Improved CTAB can isolate both satisfied RNA and DNA at same time from all the issues of Chinese jujube. 改良CTAB法在幼叶、幼嫩枝皮组织中均可得到高质量的RNA和完整的DNA,可以同时满足DNA和RNA的实验。
- The genomic DNA of 10 Actinidia species including 42 samples were extracted by the improved CTAB method. The parameters in RAPD-PCR were optimized. 以猕猴桃10个种42个样品叶片为试验材料,采用改良CTAB法提取基因组DNA,优化确立RAPD-PCR的反应体系。
- With the improved CTAB protocol, high quality genome DNA of the Lespedeza populations were obtained for RAPD amplification. 以改进的CTAB法获得了较高质量的胡枝子属植物基因组DNA,可以很好地用于RAPD扩增研究。
- Total DNA in leaf tissue of F. engleriana was extracted by four methods. Improved CTAB method was proven to be the best one. 本研究采用四种DNA提取方法,通过比较得出改良CTAB法提出的DNA纯度较高,能够达到扩增要求,因此采用此方法用于正式DNA的提取。
- Results DNA extracted with improved CTAB from fresh samples of Polygonatum odoratum was successfully amplified with PCR technique. 结果改良CTAB法提取得到的DNA可以很好地用于PCR扩增。
- RAPD analysis of 30 American hickory varieties introduced from America was carried out with leaf as material, using improved CTAB method and 16 Operpon. 以叶片为材料,采用改良CTAB法和16种Operon引物对从原产地美国引进的30个美国山核桃主要栽培品种进行RAPD分子分析。
- The genetics and variation in Actinidia were studied by analysis of genomic DNAsextracted by the improved CTAB method from 10 species including 42 biotypes. 本文以猕猴桃 10 个种、34 个栽培品种共 42 个生物型的叶片为试验材料,采用改良 CTAB 法提取基因组 DNA,对猕猴桃各生物型遗传变异进行研究。
- The improved CTAB technique can be used to extract DNA from dried samples of G. elata, but the DNA can be used only for gene amplification not for sequencing. 改良CTAB法能有效提取天麻干品DNA,提取的DNA可用于基因扩增。
- The methods of isolating total RNA from plant tissues, including Improved CTAB, SDS-phenol, Hot borate, Guanidine Isothiocyanate and Trizol kit method, were compared and improved. 改良CTAB法和改进SDS-酚法可从田间叶片中得到高质量的RNA,改良CTAB法提取的RNA纯度高于SDS-酚法,但产率却显著低于SDS-酚法;
- CTAB method,improved CTAB method,improved CTAB-high salt precipitation method were used to extract genomic DNA from the leaves of Ph. edulis. The DNA samples obtained were tested by UV spectrophotometer(3 300),agarose gel electrophoresis and PCR method. 应用CTAB法、改良CTAB法、改良CTAB-高盐沉淀法对毛竹基因组DNA进行了提取,并用紫外分光光度计(UV3300)、琼脂糖凝胶电泳和PCR分析对提取的DNA进行了检测,对DNA的产量、质量、PCR效果等方面进行了综合比较。
- Effects on isolating pear genomic DNA by using SDS,CTAB,SDS-CTAB,improved CTAB,high salt-low pH and fractional centrifugation are analyzed and compared. Seven varieties of pear are used. 以 7个梨品种为实验材料 ,比较分析了SDS法、CTAB法、SDS CTAB法、改良的CTAB法、高盐低pH值法、分步离心法对梨总DNA提取的效果。