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- immune antibody library 免疫抗体库
- AIM: To construct a anti dodecane tertraacetic acid yttrium (DOTA Y) immune Fab phage antibody library. 目的 :构建钇 十二烷四乙酸 (Y DOTA)免疫噬菌体Fab抗体库。
- Objective: To construct human tetanus immune phage antibody library, screen antibody genes against tetanus toxoid, and express solution Fab in Escherichia coli. 目的:建立人破伤风免疫噬菌体抗体库,筛选抗破伤风类毒素的抗体基因,并在大肠杆菌中表达可溶性Fab段。
- AIM: To construct phage human anti idiotypic antibody library . 目的:构建噬菌体人源抗独特型抗体库。
- The optimized strategy for the amplifying and screening of human-mouse phage Fab antibody library. 第二部分:噬菌体展示F ab抗体库构建和筛选条件的优化。
- Generation of human recombinant antibody Fab fragment to influenza virus from combined antibody library. 流感病毒中和性基因工程Fab抗体的研制
- Aim The phage display antibody library was constructed to obtain functional anti Fas Fab. 目的构建噬菌体抗体库,获得具有功能的抗Fas?Fab噬菌体抗体。
- Result The repertoire single-chain antibody library against nucleoprotein of influenza virus A were successfully constructed. 结果成功利用噬菌体表面展示技术,构建了A型流感病毒核蛋白全套单链抗体库,库容量达到82×107。
- We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library. 为了构建人源性抗脑胶质瘤抗体库，我们必须先从患者的血液内检测到抗脑胶质瘤抗体，才能为建立人源性噬菌体抗体库奠定基础。
- AIM: To construct the phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus. 目的 :利用抗SARS冠状病毒IgG抗体阳性的SARS康复患者外周血淋巴细胞 ,构建人源Fab段抗体文库。
- The phage display antibody library is developed by reasonably combining the antibody library technology and phage surface display system. 将抗体库技术与噬菌体表面展示系统有机地结合就发展成为噬菌体展示抗体库。
- The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds.The last round enriched phage clones were used to reinfect. 由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。
- Methods By genetic engineering technology,human anti-keratin ScFv was constructed from Fab fragment selected from established semi-synthetic phage antibody library. 方法利用基因重组技术对从半合成噬菌体抗体库中克隆的人源性抗角蛋白抗体Fab片段进行改造,获得了人源性抗角蛋白ScFv片段,并进行DNA序列分析。
- Conclusion: A human phage-display antibody library has been successfully constructed.The selected ScFv fragment can specifically bind to human lung adenocarcinoma cell line A549. 结论:通过噬菌体展示技术得到肺癌相关人源单链抗体,筛选后的单链抗体能与肺腺癌细胞A549特异性结合。
- This makes humoral immune antibodies not easy to identify and attack HIV. 这使得体液免疫的抗体不容易识别并攻击HIV。
- The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology. 噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法,在生物科学领域极具潜力。
- Wang Y, Hua B, Liu QY, et al.By-passing immunization: Construction of a phage antibody library with randomized CD3[J].ChinJ Microbiol Immunol, 1997, 17(6): 449-52. [5]王琰;化冰;刘群英;等.;随机化CD3抗体库的构建及不经免疫制备抗体的初步探讨[J]
- Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research. 结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体,为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
- Keywords diphtheria;immune antibody;surveillance; 白喉;免疫抗体;监测;
- The antibody library of Fab fragment from a convalescent patient infected with SARS conronavirus was constructed. The recombinant rate of light chain and heavy chain of Fd gene were 91% and 75%, respectively. 构建了源于SARS康复患者血清中抗Fab段的抗体文库 ;轻链、重链Fd段基因的重组率分别为91%25和 75%25 ;库容量为 7.;2 3× 10 7。
