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- Methods Prepare McAb by hybridoma technique,and screen by ELISA combined with immunohistochemical method. 方法采用杂交瘤技术制备单克隆抗体,并以ELISA结合免疫组化技术进行筛选。
- Used the hybridoma technique to prepare the PPPA2?P5?A2?P5?A monoclonal antibodies by immunzing Balb/c mice with PPPA2?P5?A2?P5?A coupled to BSA. 将PPPA2?P5?A2?P5?A（2?－5?P3A3）与甲基牛血清白蛋白偶联后免疫Balb/c小鼠，用杂交瘤技术获得了抗2?－5?P3A3单克隆抗体。
- Objective To prepare monoclonal antibody (McAb) against cysticorus cellulosae antigen by hybridoma technique, and to establish I-ELISA method for clinical application. 目的利用杂交瘤技术制备抗猪囊虫单克隆抗体,建立抑制性酶联免疫吸附法I-ELISA并应用于临床。
- The ideal monoclonal antibody was ought to origin from human, but which could not to come true in nowdays, because of hybridoma technique、affinity and so on. 因此，应用于临床的理想抗体应是人源的，但人-人杂交瘤技术目前尚未突破，且还存在人-人杂交瘤体外传代不稳定、抗体亲合力低及产量不高等问题。
- Methods Recombinant GDH was used to immunize Balb/C mice and the mAbs against GDH were prepared using hybridoma technique followed by identification of IgG isotype and its affinity. 方法用基因重组的GDH蛋白免疫Balb/C小鼠,采用杂交瘤细胞技术制备McAb,测定其免疫球蛋白亚类及其亲和力。
- The BALB/c mice were immunized with the whole antigen GSH-S-DNP-BSA,and hybridoma cell lines 4A4, 6A6, 1C8 and 4G3 which secreted monoclonal antibodies specific to GSH were established by lymphocyte hybridoma technique. 用全抗原免疫BALB/c小鼠，通过淋巴细胞杂交瘤技术，制备出抗GSH的单克隆抗体杂交瘤细胞系4A4、6A6、1C8和4G3等。
- With the establishment of lymphocyte hybridoma techniques,mAb develop for a long term which changed from diagnose tools to therapeutic medicine. 杂交瘤技术建立以来,单抗研究走过了漫长而又曲折的历程,分子生物学的发展使单抗从诊断工具变为有效的治疗药物。
- Using hybridoma techniques, six monoclonal antibodies (mAb), P3, 4A4 6B1, 7A6, 7D1 and 7D8 to Freesia chlorotic stripe mosaic virus ( FCSMV), were prepared. 采用杂交瘤技术,得到抗小苍兰褪绿条纹花叶病毒(FCSMV)六个单克隆抗体P3、4A4,6B1,7A6,7D1和7D8。
- the other is preparation of monoclonal antibody by using hybridoma technique. 但不论用何种技术制备的抗体都需要进行纯化。
- The technique is still at the experimental stage. 这项技术正处于实验阶段。
- Keywords primary hepatocyte;hybridoma technique;hepat-hybridoma cells;cytochrome P450;in vitro drug metabolism study; 原代肝细胞;杂交瘤技术;肝杂交瘤细胞;细胞色素P450酶;体外药物代谢研究;
- This technique is still in wide use. 这一技术仍被广泛使用。
- The technique of mapping a region or district. 地志学绘制地区地图或行政区地图的技术
- The Comparison of IL-2 and IL-6 Activity Level in a Variety of Feeder Cell Culture Supernatants and Conditioned Media for Hybridoma Technique 不同杂交瘤饲养细胞上清和条件培养液中IL-2和IL-6水平的比较
- He displayed a flawless technique in the competition. 他在竞赛中显示出了精湛的技艺。
- In many sports (physical) fitness is not as important as technique. 在许多体育运动中，体能没有技巧重要。
- A person with masterly skill or technique in the arts. 艺术名家在艺术上造诣很深的人
- In many sports physical fitness is not as important as technique. 在许多体育运动中，体能没有技巧重要。
- They have verified the applicability of this new technique. 他们已经证实了这项新技术的实用性。
- A musician with masterly ability, technique, or personal style. 演奏能手功力深厚、技巧娴熟或有个人风格的音乐师
