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- Increasing the Transient Expression of GUS Gene in Plant Tissue by Homologous Recombination. 利用同源重组提高外源GUS基因在植物组织中表达。
- AV1. SpaT-hIGF-1 was synthesized by homologous recombination of pDNR-hIGF-1 and pInt. AV1.;SpaT同源重组形成pInt
- Objective To test the anti-tumor effect of adenovirus vector generated from the homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell. 目的探讨用细菌内同源重组法制备含TK自杀基因腺病毒对肝癌细胞的杀伤作用。
- The mutant 31BK was engineered, in which homologous recombination of the aroBKanr gene cassette into the aroD locus(aroD::aroBKanr)of the E. 构建宿主菌基因精确定位突变株31BK(aroD::aroBKan~r)
- Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 酵母基因中断技术是研究酵母基因功能的重要手段,自80年代初诞生以来经历了不断的改进和发展.
- We start with these fragments, and then we have a homologous recombination system that reassembles those into a chromosome. 我们以这些片段开始,然后通过同源重组将其组合到染色体中。
- Abstract: Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 摘 要: 酵母基因中断技术是研究酵母基因功能的重要手段,自80年代初诞生以来经历了不断的改进和发展.
- Most members of the XRCC genes family participated in several DNA repair pathways, including base excision repair, homologous recombination repair and non-homologous end joining. 目前已鉴定的这一基因家族的多数成员均参与几种重要的DNA修复途径,包括碱基切除修复、同源重组修复和非同源末端重接。
- Each open reading frame (ORF) is knocked out using a PCR-based gene deletion strategy that takes advantage of the high degree of homologous recombination in yeast. 每个开放读码框使用基于PCR基因删除策略,使用在酵母中的高度同源重组优势。
- The amplified gene was cloned into the pENTR11 vector. With the pENTR11-SLC plasmid and the backbone plasmid pAd/CMV/V5-DEST, the homologous recombination reaction took place in vitro. The reaction mixture was transferred into TOP10 E. 方法采用PCR技术从含有SLC基因的质粒上扩增出SLC基因,将PCR产物酶切后连接至pENTR11载体上,再通过pENTR11与腺病毒骨架载体pAd/CMV/V5-DEST之间的同源重组作用将SLC基因片段重组至pAd/CMV/V5-DEST上,最后经293细胞的包装扩增后得到携带SLC基因的重组腺病毒。
- In S. coelicolor, the rapid increase of SCP2? plasmid conjugation efficiency and that of plasmid homologous recombination efficiency associates with development of aerial mycelium. 在天蓝色链霉菌中,SCP2质粒接合转移频率的快增长,SCP2质粒介导的质粒同源性重组频率的快增长与气生菌丝的形成同步。
- Though RNA dot hybridization is negative, OP J18-1400 has a conservative domain of seven base pairs DNA sequence: 5-TTCCCTC-3, which is a homologous recombination hotspot domain in atp6 gene. 虽然OPJ18-1400对应的RNA斑点杂交呈阴性,但其DNA序列中存在一个七碱 基5-TTCCCTC-3的保守系列,它是atp6基因中同源重组热点区,其促使线粒体基因重组形成嵌
- Diploid individuals can repair a mutated section of its DNA via homologous recombination, since there are two copies of the gene in the cell and one copy is presumed to be undamaged. 二倍个体可以通过同源重组,修复它DNA的一个突变片段,因为在细胞中有两个拷贝的基因,其中一个拷贝假定为未被损坏的。
- Objective To construct recombinant adenovirus by homologous recombination in bacteria. 目的通过细菌内同源重组方法构建重组腺病毒。
- Homologous recombination in bacteria 细菌内同源重组
- somatic homologous recombination 体细胞同源重组
- homologous recombination di-recting sequences HRDS
- homologous recombination di-recting sequences(HRDS) 同源重组引导序列
- extrachromosomal homologous recombination ECR
- extrachromosomal homologous recombination(ECR) 染色体外同源重组