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- METHOD: We extracted the RNA of Toxoplasma gondii (RH strain)by using a single step acid guanidinium thiocyanate method and isolated the poly(A) +RNA by using messenger RNA affinity membrane containing poly U to synthesize ds cDNAs. 方法:用异硫氰酸胍酸性一步法提取弓形虫(RH株)RNA,含PolyU的mRNA亲和膜分离Poly(A)+RNA,进而合成双链cDNA。
- method of guanidinium thiocyanate 异硫氰酸胍法
- guanidinium thiocyanate 硫氰酸胍
- Thiocyanate is naturally present in body fluids like blood and salia. 硫氰酸一般存在于体液中,如血液和唾液。
- The effect of solvent polarity and guanidinium on the con-formation of carbonic anhydrase was studied by fluorescence emission spectrum. 以紫外荧光光谱为检测手段,比较研究了溶剂极性及盐酸胍对碳酸酐酶构象的影响。
- The main products cover ammonuium sulfocyanide,sodium thiocyanate, potassium sulphocyanide, cuprous sulfocyanide etc,Calcium Thiocyanate. 主要产品有硫氰酸铵、硫氰酸钠、硫氰酸钾、硫氰酸亚铜、硫氰酸钙等。
- Abstract : The effect of solvent polarity and guanidinium on the con-formation of carbonic anhydrase was studied by fluorescence emission spectrum. 摘要 : 以紫外荧光光谱为检测手段,比较研究了溶剂极性及盐酸胍对碳酸酐酶构象的影响。
- These results suggest that thiocyanate may not be present in the airway surface liquid of indiiduals with CF. 这些研究结果提示在囊性纤维化患者气管表面粘液层中可能检测不到硫氰酸分子。
- In this review, recent progresses in the research of guanidinium ionic liquids are surveyed in three aspects, syntheses, properties and applications. 总结了近年来胍盐离子液体的合成、性质和应用三个方面的研究进展。
- Sodium thiocyanate(NaSCN) and dimethylacetamide (DMAC) two-step process technologies were regarded as reasonable ch. 建议选用硫氰酸钠二步法及二甲基乙酰胺(DMAC)二步法腈纶工艺。
- Guanidinium ionic liquids are concerned mainly due to their high thermal stability, chemical stability and catalytic activity. 胍盐离子液体由于具有较高的热稳定性、化学稳定性和催化活性而受到关注。
- Despite its name and its chemical relationship to cyanide, thiocyanate is not toxic. 除名称和化学结构上和氰化物有相似之处外,硫氰酸是无毒的。
- The content of NaCl in soda ash is determined by colourimetry of mercury thiocyanate. 作者采用硫氰酸汞比色法测定纯碱中氯化钠的含量.
- Township enterprises Products of coal, pig iron, castings, thiocyanate, perchlorate, mainly refractory. 乡镇企业产品以煤炭、生铁、铸件、硫氰、酸铵、耐火材料为主。
- The whitening process was performed on PAN fiber produced by sodium thiocyanate wet spinning. 对硫氢酸钠湿法二步法腈纶进行增白,探讨了主要增白工艺条件对增白效果的影响。
- Methods:To compare the method of the extract of RNA from goldfish using laminar analysis column with that of using one-step guanidinium isothiocyanate-alcohol phenyl-chloroform. 方法:应用本层析柱方法从金鱼组织所提取RNA和采用异硫氰酸胍-酚-氯仿一步法提取RNA的比较。
- Abstract The extraction properties and mechanism of niobium thiocyanate complex with secondary amine 7203 are studied. 摘要 研究了仲胺7203(简写为7203)萃取铌硫氰酸盐络合物的性能及其机理。
- Methods RNA was isolated from the human osteosarcoma cell line U-20S by single-step method of acid guanidinium isothiocyanate phenol-chloroform extraction. 方法 采用异硫氰酸胍一步法从人骨肉瘤细胞株U-2OS 中提取总RNA,以逆转录一聚合酶链反应(Reverse Transcription coupled Polymerase Chain Reaction,RT-PCR)扩增cDNA基因片段。
- Cadmium mercury thiocyanate and Zinc cadmium thiocyanate(abbreviated as CMTC and ZCTC,respectively)are two of the most important nonlinear optical crystals. 简要报道了在硫氰酸镉汞晶体和硫氰酸锌镉晶体表面镀减反射膜的研究。
- Take the average absorbance of your cauliflower homogenate and determine the thiocyanate ion concentration using the previously plotted graph. This has value of 5 marks. 利用前一题所画出来的图,将所有的花椰菜均质液的分光光度计读数(吸光值)平均,并计算花椰菜均质液中硫氰盐的浓度。此题5分。