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- The methods of isolating total RNA from plant tissues, including Improved CTAB, SDS-phenol, Hot borate, Guanidine Isothiocyanate and Trizol kit method, were compared and improved. 改良CTAB法和改进SDS-酚法可从田间叶片中得到高质量的RNA,改良CTAB法提取的RNA纯度高于SDS-酚法,但产率却显著低于SDS-酚法;
- guanidine isothiocyanate 异硫氰酸胍
- 10. The methods of isolating total RNA from plant tissues, including Improved CTAB, SDS-phenol, Hot borate, Guanidine Isothiocyanate and Trizol kit method, were compared and improved. 改良CTAB法和改进SDS-酚法可从田间叶片中得到高质量的RNA,改良CTAB法提取的RNA纯度高于SDS-酚法,但产率却显著低于SDS-酚法;收藏指正
- Mainly contain isothiocyanate as the alive compositions. 主要含有异硫氰酸盐等活性成分。
- Guanidine gum has better ability of temperature resistance and forming fissure. 清洁压裂液具有低污染、很强的抑制黏土膨胀能力、较强的携砂能力。
- Fluorescein isothiocyanate(FITC) is an important fluorescence probe. 异硫氰酸荧光素(FITC)是一种重要的荧光探针。
- A compound disinfectant contains chitin,lysozyme and polyhexamethylene guanidine as the germicidal components. 试验用复方消毒剂是以甲壳素、溶菌酶及聚六亚甲基胍等为杀菌成分。
- The conformational change courses in the different concentrations of guanidine have been to be first order reactions. 同时还测定了不同浓度胍溶液中人肌酶的失活与构象变化的速度常数。
- They were tethered with N-hydroxysuccinimide-folate and fluorescence isothiocyanate (FITC). 再将其末端用N-羟基琥珀酰亚胺-叶酸和异硫氰酸荧光素 (FITC) 联接。
- Orthogonal analysis was done about p-fluorophenyl guanidine carbonate and optimum synthesis conditions were obtained. 同时对合成对氟苯胍碳酸盐进行了正交分析,得出了最佳合成条件。
- Further study reveals phenyl isothiocyanate also react with complexes 20 and 23 to yield the insertion products. 进一步研究表明,PhNCS也可以和稀土吩噻嗪化合物反应得到相应的插入产物。
- Performances of direct extraction of gold from alkaline cyanide solution with guanidine hydrochloride were studied. 研究了盐酸胍直接从碱性氰化液中萃取金的性能。
- This could give reason for aggregate formation of GAPDH in the presence of low concentration guanidine hydrochloride. 说明在低胍作用下,酶分子发生的构象变化,导致天然态内埋疏水面的暴露,暴露的疏水面间的相互作用是形成聚合的主要原因。
- Then the inclusion bodies were redissolved by 7 mol/L guanidine hydrochloride containing 10 mmol/L of DTT. 而稀释复性法是研究其它复性方法的基础,试验中先用含2 mol/L 尿素的0.;1 mol/L Tris-HCl 缓冲液对包涵体进行洗涤,然后用含10 mmol/L DTT 的7 mol/L盐酸胍溶解包涵体。
- A new derivatization reagent,tributylsilyl isothiocyanate(TBuS ITC),is applied to C_terminal sequence analysis of peptides. 报道了一种新的用于化学降解法蛋白质C 端序列分析的偶联试剂三丁基硅异硫氰酸酯 (tributylsilylisothiocyanate;TBuS ITC) .
- We study the protein adsorption on PEU/LCP composite biomaterial surface by labeled protein with fluorescein isothiocyanate (FITC). 本研究以荧光标记为手段,研究了PEU/LCP复合生物材料表面的蛋白质吸附行为。
- His team also examined phenethyl isothiocyanate (PEITC), a constituent of cruciferous vegetables such as broccoli, on ovarian cancer cells. 报道说,这项研究或许能帮助解释为什么那些食用大量蔬菜水果的人患癌症的风险要比其他人低的原因。
- Allyl isothiocyanate (AITC) is the most important component with biological activity in horseradish.It shows functions of ant-cancer and ant-tumor. 烯丙基异硫氰酸酯(AITC)是辣根中最重要的生物活性成分,具有很高的抗肿瘤和抗癌活性,研究了AITC与哌啶、吗啉、二乙胺等胺类化合物的反应动力学和光谱性质。
- The renaturation of polyphenol oxidase from Tobacco denatured in 6 0 mol/L guanidine hydrochloride(GdnHCl) was investigated. 研究了盐酸胍变性烟草多酚氧化酶后的复性。
- Abstract : On the basis of guanidine thiocyanate method, an economical method of extracting and puring total RNA from Yunjie stigmas was established. 摘要 : 本文在常规异硫氰酸胍法的基础上,改进并确立了一种适合芸芥柱头总RNA的提取与纯化方法。