- Method:analyse its genome segment and its coding protein,then compare with its changing of surface type for infer the reason of changing.
- The full length cDNA of the genome segment (S9) of Rice gall dwarf virus Xinyi isolate (RGDV-C) was cloned and the complete nucleotide sequence was determined.
- Methods The complete nucleotide sequence of the S genome segment of Pf26 virus strain was determined with two clones, which were obtained by RT PCR,and by linking two overlapping fragments into PUC19 vector respectively.
方法 选择汉坦病毒 S基因序列保守区设计引物 ,用 RT- PCR方法将 S基因分两段扩增 ,再将扩增片段连接到 PU C19载体上 ,然后进行测序和分析。 结果 Pf2 6株的 S基因片段的全序列长为 1776 nt。
- Reovirus includes 3 serotypes.Homology of proteins encoded by S1 genome segment was less than 26% between serotypes, while that of viral proteins encoded by other 9 segments was all more than 86%.
- Genomic segment 10,11 of J19 encoding NSP4 and NSP5 were 739 bp and 649 bp in length, and the deduced proteins were 213 AA and 176 AA respectively.
J19株NSP4和NSP5基因为基因10和11,全长为739 bp 和649 bp,它们分别编码213个和176个氨基酸。
- Genomic segment 5,7,and 8 of J19 encoding NSP1,NSP2,and NSP3 were 1307bp,1004bp,and 932bp in length,and the deduced proteins were 395 aa,297 aa,and 262 aa respectively.