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- METHODS: The shRNA specific to PTI-1 (PC-3) was synthesized and inserted into pEGFP/U6 by gene recombination technology to construct the expression vector, pEGFP/U6-mPs, which was then transfected into the cultured PC-3 cells. 方法:采用基因克隆技术,将合成的短发卡样特异性PTI1(PC3)RNA干扰寡核苷酸序列插入真核表达载体pEGFP/U6,构建PTI1(PC3)shRNA的真核表达载体,体外转染人前列腺癌PC3细胞,48h后观察细胞生物学变化;
- Meanwhile, the applications of gene recombination technology and cell culture technology in preparation of resveratrol were explained systematically, and its application prospect was also viewed. 同时对基因重组技术、细胞培养技术在白藜芦醇制备方面的应用作了系统阐述,并对其应用前景进行了评价。
- gene recombination technology 基因重组技术
- Site-specific recombination technology provides an important means for regulating transgene expression and improving efficiency of gene transformation. 摘要定点重组转基因技术是调节外源基因表达，提高转基因效率的重要手段之一。
- Gene recombination and gene transfection are the new progresses in molecular biology,which provides theory and technology basis for further study of tissue engineering. 基因重组、基因转染是分子生物学研究的新进展,为组织工程的深入发展提供理论和技术基础。
- DNA splicing model is a grammar system based on the biological DNA molecular recombination technology. DNA剪接计算模型是以生物DNA分子重组技术为基础的文法系统。
- Recombinant fragments;recombinant technology. 重组DNA碎片;重组DNA技术
- IBV is a sRNA virus,the gene of the virus always mutated owing to it's point-mutation and gene recombination, so the virus have many serotypes. IBV是单股RNA病毒,该病毒基因可因点突变和重组而发生变异,故传染性支气管炎病毒血清型较多。
- Now focus on the research of gene recombination anti-tumor polypeptide and the basic research of polypeptide for curing bone fracture、osteoporosis. 近期研究领域侧重于基因重组抗肿瘤多肽和治疗骨折、骨质疏松多肽的应用基础研究。
- The antibody antihalf-antigen and antigenic can be mass-produced by gene recombination and used in RII for clinical immunologic diagnosis and scientific researches. 利用基因重组技术大量生产的任何一种相应半抗原和抗原的抗体片段,与RII技术联合,已应用于临床免疫诊断和科学研究。
- This code can be applicable to the design, construction and acceptance of newly built, rebuilt and enlarged biosafety laboratories that used for microbiology, biomedicine, gene recombination and biological produce. 本规范适用于微生物学、物医学、物实验、因重组以及生物制品等使用的新建、建、建的生物安全实验室的设计、工和验收。
- An improved immune genetic algorithm has been put forward in this paper.It is based onthe thoughts of vaccine selection, vaccination, immune memory, gene affinity mutation, gene recombination. 摘要本文借鉴疫苗提取、疫苗接种、免疫记忆、基因亲和突变、基因重组等思想提出了一种改进的免疫遗传算法。
- Recombinant technology applied to the bacterium can produce altered hemoglobin molecules that do not break down into half molecules. 应用于细菌的重组技术，能够产生不分解成半分子的可变化的血红蛋白分子。
- Recently,by taking advantage of the vector-specificity and using gene recombination,small RNA chains,vectors or other microsomes were chemically modified to enhance the tissue-specificity. 为了增强对组织靶向性的治疗应用,目前研究有利用载体的特异性及基因重组构建进行调控作用于靶组织,也可以小分子基团特异性化学修饰小RNA双链或载体及其他微粒等方法提高组织靶向性。
- The result indicates that recombinant PCR is a very effective and reliable method for gene recombination in vitro . 重组 PCR技术是十分有效、可靠的体外基因重组方法
- DNA recombinant technology have brought innovation to the biolog y technology, it can supply the strains with strong selectivity and ability of accumulating target metabolites. 重组DNA技术使生物技术取得了彻底性的变革，它可以提供具有极强选择性和累积目的代谢物能力的菌株。
- The green fluorescence protein (GFP) gene recombinant virus labeling is a new method for the neuroanatomical studies. 绿色荧光蛋白(GFP)基因重组病毒标记技术是神经解剖研究的新方法。
- Gene Recombination and Genetic Engineering 基因重组与基因工程
- Gene recombination human growth hormone 基因重组人生长激素
