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- Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 酵母基因中断技术是研究酵母基因功能的重要手段，自80年代初诞生以来经历了不断的改进和发展.
- Abstract： Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 摘 要： 酵母基因中断技术是研究酵母基因功能的重要手段，自80年代初诞生以来经历了不断的改进和发展.
- The gene function was studied by using the method of gene disruption and the result showed that the sawl played an important role in developmental changes from aerial hypha to spore formation of Streptomyces ansochromogenes. 用基因破坏的策略进一步研究了该基因的生物学功能;结果表明saw1在圈卷产色链霉菌气生菌丝到孢子形成的发育转变中有重要作用;是分化中控制孢子发育起始的一个重要基因.
- The vector of narG gene targeting pEGude was introduced into denitrifier DNF409 by electrotransformation. An Erm~r Cm~s isolate DNF409EF was obtained and narG gene disruption was confirmed by PCR. 将基因中断载体pEGude电激转化至反硝化菌DNF409中，通过抗性筛选得到表型为Erm~r Cm~s的重组子DNF409EF，并通过PCR分析证实narG基因已被正确中断。
- PCR-mediated gene disruption technique makes the manipulation easi er and it can be used to precisely delete genes in yeast. PCR介导的酵母基因中断技术，大大简化了操作，实现了酵母基因的精确缺失；
- Research Advances of Yeast Gene Disruption 酵母基因中断技术研究进展
- Keywords Geldanamycin;Biosynthetic gene;Genomic library;Gene disruption; 生物合成基因;基因文库;基因阻断;
- Keywords Streptomyces tenebrarius;carbamoyltobramycin;the biosynthetic gene （cluster）;gene disruption; 黑暗链霉菌;基因阻断;接合转移;基因突变;
- Keywords quinic acid,biosynthesis,shikimate pathway,heterogenous enzyme,co-expression,gene disruption,gene integration; 奎尼酸;生物合成;莽草酸途径;异源酶;串联表达;基因破坏;基因整合;
- Keywords Daunorubicin;4'-epi-Daunorubicin;C4 Ketoreductase;Gene Disruption;Function Compensation; 柔红霉素;表柔红霉素;C4酮基还原酶;基因阻断;功能补偿;
- Keywords dnmV Daunorubicin Homologous recombination Gene disruption Function compensation; 柔红霉素;同源重组;基因阻断;功能补偿;
- Keywords S.tenebrarius;Apramycin;Biosynthesis genes;Gene disruption; 黑暗链霉菌;安普霉素;生物合成基因;基因阻断;
- Keywords Amycolatopsis orientalis HCCB10007;Vancomycin;Protoplast;Transformation;Gene disruption;Streptomyces griseus IFO 13350;A-factor;Grixazone;Biosynthesis.; 东方拟无枝酸菌HCCB10007;万古霉素;原生质体;转化;基因破坏;灰色链霉菌;A因子;黄色色素:生物合成;
- The position that a given gene occupies on a chromosome. 基因座在一条染色体上某种给定基因所占的位置
- Half of the people who inherit the gene express it. 半数有这种遗传基因的人，皆显示出其影响
- Now the variant of each gene is called an allele. 每一种基因的变化就叫做等位基因。
- We know a lot about the structure of gene now. 如今我们对基因的结构有了较多的了解。
- A single gene may have many effects. 单一基因可能具有很多种效应。
- There can be more than two alleles for a gene. 一个基因可能有两个以上的等位基因。
- A mutable organism; a mutable gene. 一个易变的生物；一个易变的基因
