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- gene deleted live vaccine 基因缺失活疫苗
- the gene mutagenesis was used to construct MTB attenuated live vaccine such as MTB purine autotroph, ICL gene deletion,VitB 5 autotroph and undecaprenol kinase mutant. 利用基因诱变可构建 MTB减毒活疫苗,如MTB嘌呤营养缺陷株, ICL基因剔除株,VitB5营养缺陷株和十一萜 醇激酶基因变异株。
- First killed vaccine was used to prevent Salmonella,then gene engineering attenuated live vaccine was used step by step. 最早用灭活苗来预防沙门菌病,逐步发展到基因工程减毒活疫苗,其中基因工程活疫苗经过不断的发展,逐步显示出其优越性。
- So, CAV-2 has potentiality to develop into live vaccine vector. 因此,CAV-2具有发展成为活疫苗载体的潜力。
- A live vaccine made from HIV, he maintains, can be made safer by removing not just the nef gene but several others as well. 他主张可以把HIV病毒中的几种基因与“否因”一并移除，就能增加活疫苗的安全性。
- LacZ gene was inserted into TK gene deleted transfer vector, the TK /LacZ+ /GTPV-TY mutant was screened. 将LacZ基因表达盒插入TK基因缺失转移载体,筛选TK基因缺失的GTPV-TY突变株。
- However, when that antibody is almost gone, the chick becomes susceptible and must be immunized with live vaccine. 当母抗将要消失，鸡只变得敏感时必须使用活疫苗对雏鸡进行免疫。
- A single copy fragment containing cis acting "CACA" box has been identified, which might be the upstream of a new tumor suppressor gene deleted in tumors of the stomach and small intestine. 在胃、小肠肿瘤组织发生缺失突变的片段可能为某一抑癌基因的前半部分 ,即含有启动子成分的调控元件部分CACA盒
- A PCR-ELISA method was established to detect Mycoplasma in live vaccine , and to inquire the optimum experiment conditions. 建立了检测兽用疫苗中支原体污染的PCR-ELISA方法,探索其最佳实验条件及优化组合。
- Results: three patients with DAZ gene deleted were found, who are all idiopathic azoospermia with normal karyotype. It accounted for 14.3%(3/21) of idiopathic azoo- spermia, which was in accordance with the public reports (5~16%). 结果：PCR结果共检测出3例DAZ基因缺失，均为核型正常的特发性无精患者，约占特发性无精患者的14.;3%25(3/21)，符合文献报道的5~16%25。
- Results It was revealed that the live vaccine strain of F. tularensis induced the expression of Tfr1 in host macrophages. 结果土拉弗朗西斯菌疫苗株可以诱导转铁蛋白受体-1在宿主巨噬细胞中表达。
- Objective To shorten the observing time of the antibody attenuation trend after the measles live vaccine being inoculated,a power curve model of antibody level trend has been established and the antibody permanence been prospected in the paper. 目的建立接种麻疹减毒活疫苗(MV)后抗体水平变化趋势的幂曲线模型,预测抗体水平的持久性,缩短接种MV后抗体免疫持久性的实际观察时间。
- Results: The inoculation rates of BCG, whole range of HBV vaccine, poliomyelitis live vaccine, DPT, measles vaccine live,and vaccinum encephalitis B were 97.0%, 90.6%, 76.0%, 80.9%,88.6%,81.3% respectively. [结果]卡介苗接种率97.;0%25;乙肝疫苗全程接种率为90
- This method was used to detect Sendai virus in Japanese encephalitis attenuated live vaccine and the kidney of nurturing hamster, which was used for producing vaccine in China for years. 将该方法用于检测乙脑减毒活疫苗和用于生产疫苗用的普通级乳地鼠肾中的Sen dai病毒。
- Objective To analyse the locations and types of gene deletions in Duchenne/Becker muscular dystrophy(DMD/BMD). 目的分析迪谢内/贝克肌营养不良症（DMD/BDM）基因缺失类型及其分布规律。
- Approximately 59% of the isolated strains of NDV represented the live vaccines. 对不同禽类（鹅、鸽、鸵鸟、鸡）中分离的NDV的测定结果表明，59%25为残留的疫苗毒。
- Objective To detect dystrophin gene deletion of Duchenne muscular dystrophy (DMD) and make DMD gene diagnosis. 目的探讨Duchenne型肌营养不良症（DMD）患者基因缺失的突变特点并进行基因诊断。
- An attenuated strain of Porcine Epidemic Diarrhea Virus(PEDV),PEDV G1P83,and a attenuated strain of Transmissible Gastroenteritis Virus of Swine (TGEV),TGVE AGI,were used for production of a condition of a combined live vaccine ofTGE andPED. 用猪流行性腹泻 (PED)弱毒疫苗株 PEDV- G1P83和猪传染性胃肠炎 (TGE)弱毒疫苗株 TGEV-AG1制成 PED和 TGE弱毒二联疫苗 ,并进行了保存期试验、安全效力、免疫期试验和区域试验。
- Results: all of 12 CML cases were bcr/abl gene positive by FISH and 2 cases were associated with ASS gene deletion. 结果:12例CML均检出bcr/abl基因的存在,其中2例伴ASS基因缺失。
- As compared with the FBA, the performance of EFM analysis was superior to FBA in prediction of gene deletion phenotype. 通过EFM预测了基因突变后的酵母细胞生长现象,模拟预测结果和实验结果很好吻合;
