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- gel shift binding assay 凝胶移位结合试验
- The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. 电泳凝胶迁移分析是一个相对简单和灵敏的研究蛋白:DNA相互作用的方法。
- Binding assay shown that Vp28 can not binding membrane protein of shrimp gill. 纯化的VP28与对虾鳃细胞膜的结合实验表明,VP28不能与对虾鳃细胞膜结合,即纯化的VP28不具有结合活性。
- The dopaminergic receptors were measured with the radio-ligand binding assay. 采用同位素标记法测定受体变化;
- Competitive protein binding assay indicates the level of cAMP is improved when CDCA is given by oral administration. 通过竞争性蛋白质结合分析法测得,鹅去氧胆酸经灌胃给药后能显著提高大白鼠气管组织中cAMP的含量。
- SSR was detected by radioligand binding assay(RBA) and ER, PR by LsAB method in these groups. 采用RBA法测定各组织的SSR表达 ,采用免疫LsAB法测定ER、PR表达。
- The effects of l-CSL on recombinant D_1R were investigated by [3~H]SCH23390 binding assay and cAMP assay. [~3H]SCH23390受体结合和cAMP测定实验检测左旋氯代斯阔任对重组D_1受体的激动作用。
- Method H?3?cAMP was used in protein binding assay to determine the level of cAMP in bovine BTC. 方法运用蛋白竞争结合法用氚(H3)标记的环磷酸腺苷(adenosine3,5?cyclicmonphos?phate,cAMP)测定牛眼小梁细胞内cAMP的含量。
- Methods: The number and affinity of IR on hepatocyte membrane of ASR were determined with radioligand binding assay. 采用放射配体结合分析方法检测肝细胞膜胰岛素受体的数目与亲和力。
- Methods Radioligand binding assay was used to determine the contents of GR and its affinity (Kd). 方法应用胃泌素受体的放射配基结合分析法,测定34例胃癌组织胃泌素受体的含量及其亲和力。
- Competitive inhibition assay, saturation binding assay and scatchard analysis testify the expression of LH/hCG receptor. 竞争结合实验、受体结合的特异性实验、饱和结合实验证明3AO细胞上存在特异性的Ll壬七CG受体。
- Methods: Wrap restraint stress induced defecation rats, the guanylin contents in colon and plasma were measured by radioligand binding assay. 方法:建立束缚应激诱发排便异常大鼠动物模型,应用放射配基法测定结肠组织及血浆中鸟苷素含量。
- In PMP radio binding assay of two different 3HPAF concentrations,each specific binding inhibition effect of SY were dosage depenent also. 两种浓度3HPAF作为配体进行PMP受体结合实验,不同浓度的红花总黄色素抑制3HPAF与受体的特异性结合均可见明显的量效关系。
- RESULTS In WRP radio binding assay of three different 3HPAF concentrations,each specific binding inhibition effect of SY were dosage dependent. 结果3种浓度3HPAF作为配体进行WRP受体结合实验,不同浓度的红花总黄色素抑制3HPAF与受体的特异性结合均可见明显的量效关系;
- The binding activity of various CsA analogs was shown as following:CsA>CsC>CsD>CsB.CONCLUSION The cyclophilin A binding assay is sensitive,reproducible and stable. 对几种CsA类似物与CyPA的亲和力进行测定的结果表明 ,其亲和力CsA >CsC >CsD >CsB。
- Our conclusion is that by competitive inhibition assay, saturation binding assay and Scatchard analysis, LH/hCG receptors with high affinity are present on 3AO cells. 结论: 竞争结合实验、特异性结合实验、饱和结合实验表明 3AO细胞膜上存在高亲和力的LH/hCG受体。
- The super helix structure of GFM3P1 and GFM3P2 is easily destroyed by 0.2mol/lNaOH, but that of GFM4P2 is hardly destroyed by 2mol/L NaOH in the Congo red binding assay. 经刚果红结合实验表明,GFM3P1、GFM3P2的超螺旋结构容易被0.;2mol/L NaOH破坏,而GFM4P2则相反,其超螺旋结构不能被2mol/L NaOH水溶液破坏。
- gel shift assay 凝胶移动检测
- The adrenoreceptor density of peripheral lymphocyteswere measured by radio ligand binding assay,and the content of interleukin-5(IL-5) and interleukin-12(IL-12) were measured by enzyme-linked immunosorbnent assay. 用放射性配基结合分析法测定外周血淋巴细胞受体的变化,测定血清白介素5(interleukin-5,IL-5)、白介素12(interleukin-12,IL-12)含量。
- An hour later they came off the night shift. 一个钟头之后他们下夜班了。