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- The rMTG was refolded by gel filtration chromatography, The specific activity and the protein concentration of MTG were 21.3U/mg and 75mg/L. 用凝胶过滤色谱法对rMTG进行了体外复性，最终得到了复性蛋白浓度为75mg/L和比酶活为21.;3U/mg蛋白的结果。
- The sample was heated at different temperature for different time, and then studied by SDS-PAGE and Gel Filtration Chromatography. 结果人参蛋白随着加热温度的升高而发生聚集和解聚;
- The changes of the molecular weight distributions of xylan during enzymatic hydrolysis have been investigated with gel filtration chromatography. 采用凝胶过滤色谱法研究了木聚糖在酶解过程中分子量分布的变化。
- XL2 was purified by three steps including breaking cell wall,ammonium sulfate precipitation,and gel filtration chromatography(GFC). 方法探讨了不同破壁方法和硫酸铵盐析提取粗酶的条件。
- High performance gel filtration chromatography (HPGFC) showed that the molecular weight of oyster glycogen was about 1.6million in the range of 10~6~10~7(Dalton). 高效凝胶过滤色谱(HPGFC)分析表明,制备得到的牡蛎糖原的相对分子质量大约为130万Dalton,与凝胶过滤色谱得到的牡蛎糖原相对分子质量在数百万至数千万之间的结果吻合。
- The molecular weight distribution of oligo-peptide in soybean protein enzymolysis products was determined through ultrafiltration combined with gel filtration chromatography(GFC). 研究采用超滤法与凝胶过滤色谱(GFC)相结合实验方法就大豆蛋白酶解产物寡肽混合物的分子量的分布状况进行了测定。
- Utilizing heat treatment and gel filtration chromatography on Sephadex G-150, antihypertensive factor (AHF) was partially purified from erythrocytes of essential hypertensive subjects. 本研究利用热处理和Sephadex G-150凝胶过滤层析等方法,从原发性高血压病患者(EHS)红细胞中部分纯化了抗高血压因子(AHF)。
- We cloned the dok1 and dok5 full-length cDNA and expressed their PTB domains, and then the recombinant proteins were purified by affinity, anion exchange and gel filtration chromatography. 我们克隆表达了dok1和dok5蛋白，并表达纯化了dok1和dok5的PTB结构域。 经Biacore实验发现，dok1 PTB结构域可特异识别来自Ret的磷酸肽，但不与Shc和Irs1 PTB识别的TrkA和IL-4R磷酸肽结合；
- The rapeseed peptides (RSP-R) ,produced with Alcalase and Flavourzyme, was separated by Sephadex G-25 gel filtration chromatography, and three parts of peptides (RSP-1RSP-2 and RSP-3) were obtained. 双酶分步水解的菜籽清蛋白粗品肽(RSP-R)经SephadexG-25凝胶层析柱分离纯化,得到分子量依次降低的三个级分(RSP-1,RSP-2和RSP-3)。
- The enzyme was purified by batch-wise adsorption and elution on DEAE-cellulose, Butyl-Toyopearl 650M hydrophobicchromatography and Sephadex G-100 gel filtration chromatography, The purityof the enzyme was increased by 66.8-fold. 脂酶提纯时;采用DEAE-纤维素搅拌吸附与洗脱、Bulyl-Toyopearl650 M疏水层析及交联葡聚糖G-100凝胶过滤层析工艺;可提高酶纯度66.;8倍。
- In this paper, a procedure of isolation and purification of human serum high density lipoprotein (HDL) by dextran sulfate precipitation in combination with gel filtration chromatography was reported. 本文用DS和Ca~(++)沉淀法结合凝胶过滤法分离纯化了人血清HDL。
- Gel filtration chromatography of tumor extract demonstrates a single major peak of bone resorption activity (BRA, 45Ca release from calvarial bone) that elutes with an apparent MW of about 15,000 dalton. 肿瘤提取液经ultrogel层析,发观仅有一溶骨活性峰(~(45)Ca自乳鼠顶骨培养中的释出率),相当于表观分子量15,000道尔顿。
- The mature Q49D-PLA2 mutant was obtained by Hitrap SP cation exchange and Superdex 75 gel filtration chromatography,with the recovery rate of 1.3%,and the specific activity of the mature Q49D-PLA2 mutant was 72 U/mg. 突变体融合蛋白经蛋白水解酶Factor Xa酶切后;采用Hitrap SP阳离子交换层析和Superdex75凝胶层析进一步纯化;得到突变体蛋白Q49D-PLA2;得率为1.;3%25;比酶活为72U/mg。
- gel filtration chromatography(GFC) 凝胶过滤层析, 凝胶过滤色谱法
- gradient gel filtration chromatography 梯度凝胶色谱
- PREPARATION OF OLIGOSACCHARiDES BY GEL FILTRATION CHROMATOGRAPHY 凝胶色谱法制备寡糖
- Keywords arabinoxylan;WEAX;xylanase;gel filtration chromatography; 阿拉伯木聚糖;WEAX;木聚糖酶;凝胶过滤色谱;
- packing for high performance gel filtration chromatography 高效凝胶过滤色谱填料
- A Study on the Refinement of Gardenia Yellow with Gel Filtration Chromatography 凝胶层析法精制栀子黄色素的研究
- Separation and purity of the soybean peptide by gel filtration chromatography 凝胶过滤法分离纯化大豆蛋白肽
