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- Of the 25 cases of ES/pPNET, 19 were positive for EWS FLI1 fusion transcript and 1 for EWS ERG fusion transcript. 80%25 ( 2 0 / 2 5 )的ES/pPNET中表达EWS FLI1或EWS ERG ;
- Among 10 cases of ES/PNET, the EWS-FLI1 fusion transcript was detected in 8 by RT-PCR. 10例ES/PNET中FISH观察9例有EWS基因的易位。
- The fusion transcript becomes a mature functional transcript after intergenic splicing. 融合转录本经基因间剪接而成为有功能的成熟转录本。
- EWS-FLI1/ERG fusion transcript was detected in 20 of the 25 ES/pPNET cases (80%) . The 15 non-ES/pPNET control cases were negative for EWS-FLI1/ERG fusion transcript. 25例ES/pPNET中20例检测到EWS-FLIl/ERG融合基因的表达(80%25),15例对照组均未检出EWS-FLIl的表达。
- EWS/pPNET and DSRCT exhibit similar features in morphology and immunohistochemistry. Detection of EWS-FLI1 fusion transcript in paraffin-embedded tissues of EWS/pPNET by RT-PCR is a reliable marker for molecular diagnosis. EWS/pPNET与促结缔组织增生性小圆细胞肿瘤(DSRCT)具有重叠的形态学和免疫组化特点,石蜡包埋组织中检测EWS-FLI1融合基因的表达可作为诊断EWS/pPNET的可靠指标。
- In both cases CCA demon- strated a complex translocation,t(6;8;21) (p22;q2;q22),which was confirmed by interphase and meta- phase FISH and AML1/ETO fusion transcript was detected by RT-PCR. 2例患者常规细胞遗传学分析显示均存在 t(6;21;8)(p22;q22;q22),间期和中期 FISH 证实了核型结果; RT-PCR 检测到 AML1/ETO 融合基因转录本;
- Methods Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect AML1/ETO fusion transcript of 36 AML M2 patients and analyze these combined with clinical manifestations. 方法 通过逆转录酶 /聚合酶链反应 (RT PCR)方法扩增M2型患者的AML1/ETO融合mRNA ,并结合临床资料综合分析。
- EWS-FLI1 fusion transcripts were detected by nested RT-PCR. EWS -FLI1融合基因检测方法采用嵌套式RT- PCR法。
- variant type of fusion transcript 变异型融合转录本
- AML1-ETO fusion transcripts were not detected in cases of t (8; 21) negative group. t(8:21)‘组，CD19、CD34的表达明显高于t(8:21)一组(p<0.;05)，CD19、34共表达率为58
- The expression of EWS-FLI1/ERG mRNA were detected in 20 cases of ES/pPNETs(80.0%), No other fusion transcripts were detected. 4例腺泡状软组织肉瘤均为ASPL-TFE3融合基因mRNA阳性表达。 运用CISH技术在66.;7%25的(10/15)的10例骨外ES/pPNETs中检测到EWS基因易位阳性信号;结果与本组病例中特异性易位融合基因的检测结果相一致。
- AML1-ETO fusion transcripts positive were found in all patients with t (8;21) chromosome, 3 cases without t (8;21) chromosome and 2 cases undetected by Cytogenetic analysis. t(8:21)‘AML组12例伴t(8:21)染色体易位者、3例无t(8:21)异常核型者一和2例未行染色体分析者，AMLI一ETO融合基因均为阳性，而对照组28例患者无一例阳性。
- The t(11;22) were detected in 10 of 15 (66.7%) ES/pPNETs by CISH technique that consistent with the results of EWS-FLI1/ERG fusion transcripts detection. pPNET、骨外尤因肉瘤和Askin瘤三者在病理形态、免疫表型、超微结构等方面存在相当程度的重叠表现。 2、骨外ES/pPNETs的免疫诊断应联合CD99/FLI1、vim、NSE和其它神经标记如S-100、Syn、CgA等标记物。
- A transcript of the tape is presented as evidence in court. 录音带的文字记录被作为证据在法庭上出示。
- RT-PCR is a feasible method for the detection of EWS-FLI1 fusion transcripts in FFPE tissues in PNET and the result is meaningful in differential diagnosis and prognostic evaluation of the tumor. 在外周原始神经外胚叶瘤石蜡包埋组织中,用嵌套式RT- PCR检测EWS- FLI1融合基因是可行的方法,检测结果对肿瘤鉴别诊断和预后判断有意义。
- I cannot vouch for the correctness of the transcript of proceeding. 我不能担保诉讼的官方记录是正确的。
- Americans are the fusion of many different races. 美国人是各种种族人的熔合体。
- Brass is formed by the fusion of copper and zinc. 黄铜是由铜和锌熔合制成的。
- A transcript of the tapes was presented as evidence in court. 录音带的文字记录被作为证据在法庭上出示。
- She went through the transcript carefully, to eliminate all errors from it. 她把誊本从头至尾认真地看了一遍，以消除其中的错误之处。
