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- HSP70-1 genotypes were determined with polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP). 采用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)方法检测HSP70-1多态性。
- Rep-PCR and restriction fragment length polymorphism (RFLP) were used to analyze the genetic structure in a population of 143 Xanthomonas oryzae pv. 用Rep-PCR和RFLP两种方法分析了143个水稻白叶枯病Xanthomonas oryzae pv.
- Objective: To establishi an amplified fragment length polymorphism (AFLP) reaction system with Gaistrodia elata Blume as material. 摘要目的:以天麻为试验材料,建立扩增酶切片段长度多态性(AFLP)分析体系。
- Method 32 cases of hydatidiform mole were analyzed by DNA restriction fragment length polymorphism (RFLP, hybridized with the probe 33.15). 方法采用DNA限制性片段长度多态性分析的方法研究32例葡萄胎标本。
- Phylogenesis of 48 tobacco germplasms from different countries or regions was analyzed with amplified fragment length polymorphism (AFLP). 摘要利用AFLP技术,对48份不同类型和地域来源的烟草种质的亲缘关系进行了分析。
- AFLP(Amplified Fragment Length Polymorphism)analysis system was established with 12 apple rootstock genotypes(Malus). 以12个苹果砧木基因型为试材,建立了扩增酶切片段长度多态性(AFLP)分析体系。
- Objective:To establishi an amplified fragment length polymorphism(AFLP)reaction system with Gastrodia elata Blume as material. 目的:以天麻为试验材料,建立扩增酶切片段长度多态性(AFLP)分析体系。
- The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP). 用聚合酶链反应-限制性片段长度多态性方法,分析限制性酶切位点-48A/的多态分布。
- Amplified fragment length polymorphism (AFLP) analysis of 20 wild and 5 cultivated strains of Agaricus bisporus (Lange) Imbach was done in terms of genetic diversity. 应用AFLP指纹技术对双孢蘑菇的20个野生菌株和5个栽培品种的遗传多样性进行了研究.
- Thirty-seven accessions of wild Dactylis glomerata L. were used in assessment of genetic diversity by means of amplified fragment length polymorphism (AFLP). 摘要应用扩增片段长度多态性(AFLP)技术,从64个引物对中筛选出9个,分别对37份鸭茅种质进行扩增,共得到400条清晰条带。
- In this study, a technique describing aquatic bacterial communitystructure has been developed by using restriction fragment length polymorphism of 16S ribosomal RNA gene (rDNA). 用细菌16S核糖体RNA基因(rDNA)限制性片段长度多型性描述了水环境细菌群落结构。
- Takin, Cattle, Asiatic black bear DNA fingerprinting was constructed with AFLP (Amplified Fragment Length Polymorphism) and with no isotope in our laboratory. 摘要用AFLP(扩增片段长度多态性)方法来构建羚牛、家牛和黑熊的DNA指纹图谱,并结合国内实验室的具体情况,避免使用同位素;
- Method Polymerase chain reaction and restriction fragment length polymorphism were used to examine 40 oral malignant tumors for loss of heterozygosity at chromosome 3p. 方法应用聚合酶链反应限制性片段长度多态性分析方法对40例口腔鳞癌组织中3号染色体的等位基因杂合缺失进行测定。
- It isconsisted of 115 simple sequence repeat (SSR) markers, 84 random amplified polymorphicDNA (RAPD), 47 restriction fragment length polymorphism (RFLP) markers, 7 isozymes, and 3morphological markers. 构建了一个具有256个遗传标记,分布较好的完整的分子连锁图谱,其中包括115个SSR标记、84个RAPD标记、47个RFLP标记、7个同功酶标记和3个形态标记。
- Amplified Fragment Length Polymorphism (AFLP ) is considered as the most powerful DNA fingerprinting technique for its high reliability and powerfu l approach in detecting polymorphism loci. 扩增片段长度多态性(AFLP)可靠性强,多态检出率高,因而被认为是最有效的DNA指纹分析技术。
- In the F 2 population from npa 1/Sheng47 the gene for the non palea trait was mapped between two restriction fragment length polymorphism markers, C498 and RZ450, with distances of 7.5cM and 2.4cM, respectively. 选用突变体为父本、生 4 7为母本杂交的F2 群体作定位群体 ,利用SSLP标记和RFLP标记将与突变性状相关的基因定位在第 6染色体短臂上RFLP标记C4 98和RZ4 5 0之间 ,暂定名为npa 1。
- Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). 方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
- Methods The technology of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect MTHFR gene C677T polymorphism in 74 HD patients and 30 healthy controls. 方法 利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法检测了首都医科大学附属北京同仁医院肾内科74例HD患者和30例健康者MTHFR C677T基因多态性。
- Methods: Polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) was used to detect the MTHFR gene C677T polymorphism in 53 normal controls and 182 infertile males. 方法:应用聚合酶链式反应-限制性片断长度多态性(PCR-RFLP)方法检测53例健康可育男性和182例不育男性的MTHFR基因C677T位点多态性。
- Methods Polymerase chain reaction combined with restrictive fragment length polymorphism was used to detect the M235T variants of AGT gene in 111 CHF cases,accompanied with 110 healthy controls. 方法应用聚合酶链反应-限制性片段长度多态性技术检测2004年4月至2005年12月珠海市3家医院的111例CHF患者和110名健康对照者的AGT基因M235T多态性并进行统计学比较。