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- fluorescent staining technique 荧光着色技术
- Methods Double labeling immunocytochemical staining technique was used. 方法采用免疫细胞化学双重标记技术。
- One is a Hoechst dye staining technique to look for extranuclear DNA spots. 在国外的生物论坛上也有很多人在讨论black dots 和black particles，也就是我们所说的黑胶虫。
- Method Immunocytochemical staining technique was used in the present study. 方法免疫组织化学染色技术。
- Methods To reproduce the PFM with surface stain technique and watch results. 方法:对烤瓷冠采用外源性染色技术进行再加工,观察临床效果。
- Methods Indirect fluorescent staining, FCM analysis and RT PCR were employed to detect the expression and regulation of PTA1 at the levels of PTA1 protein and PTA1mRNA. 用RT PCR方法检测内皮细胞中PTA1mRNA的表达 ;
- fluorescent antibody staining technique 荧光抗体染色技术
- Nonspecific and specific phagocytic activities of macrophages with heterogeneous fluorescent staining were observed using starch polysaccharide and immune activators (diphtheria toxoid and BCG) to induce and activate mouse peritoneal macrophages. 利用淀粉多糖和免疫促进剂（白喉类毒素和卡介苗）诱导和活化小鼠腹腔巨噬细胞，观察了四种异质性荧光染色的巨噬细胞非特异性和特异性吞噬活性。
- Studies on the nuclear staining techniques for Rhizoctonia spp. 丝核菌细胞核染色技术的研究。
- Immunoreactivities for Fas, FasL and caspase - 3 in-creased considerably in the cervical carcinoma tissue. Dual positive fluorescent staining for ap-optosis and caspase - 3 or Fas was appeared in some cells. 癌组织中，Fas、FasL、caspase－3蛋白阳性细胞明显增多，双重荧光染色显示，部分细胞Fas及cspase－3蛋白与凋亡细胞同时阳性染色。
- MTT method was employed to detect the proliferation of cells,while the adhesive ability was investigated by stereology. In the meantime,the cell integrity and the interface between biomaterial and cells were observed by fluorescent staining and SEM. 采用MTT法检测细胞增殖活性,体视学方法检测细胞粘附能力,荧光双染法检测细胞完整性,扫描电镜观察细胞-材料界面。
- VSMCs were lysed by cell lysis buffer and the concentration of protein was measured using Coomassie blue G250 staining technique. 裂解细胞，考马斯亮蓝G一250染色法测定蛋白浓度，使用节触stern Blotting法检测
- Methods For 23 cases (38 eyes) diagnosed of keratoconjunctivitis sicca, conjunctival impression cells were examined by using acridine orange fluorescent staining, the results being evaluated and graded by Tseng Classification. 方法选自我院眼科门诊已确诊的干燥性角结膜炎患者23例(38眼),用丫啶橙荧光染色结膜印迹细胞检查法进行检测,并用结膜印迹细胞检查结果评价标准进行评价,按Tseng分类法进行分级。
- Objective To evaluate the method of study on lymphatic capillaries in the liver using the immunohistochemical staining technique. 目的评价免疫组织化学染色法在肝脏淋巴管研究中的作用。
- Methods: IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index (DI) and SPF were calculated. 方法:将IRE-EGFP-esp1质粒用Fu-GENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系(esp1-A549)。 采用DNA荧光染色流式细胞仪分析转染后细胞DNA含量、倍性及细胞周期,计算S期细胞比率(SPF)和DNA指数(DI);
- Objective Explore the value of APM histochemical stain technique in the diagnosis of amniotic fluid embolism(AFE). 目的探讨APM染色法在羊水栓塞(AFE)病理学诊断中的应用价值。
- Aim To evaluate the functional condition of corneal cells and to detect the degree of keratoconjunctivitis sicca using conjunctival impression cell test with acridine orange fluorescent staining. 目的应用丫啶橙荧光染色法进行结膜印迹细胞学检查,通过判断结膜细胞功能状态,对干燥性角结膜炎患者的眼部干燥程度进行定量检测,为临床的治疗提供实验室依据。
- Methods Twenty three patients with serious blepharitis were given slit-lamp examination, tear break-up determination, Schemer 1 test and corneal fluorescent staining before and after drug therapy. 方法对23例严重睑缘炎患者进行详细裂隙灯检查、泪膜破裂时间、泪液分泌试验测定及角膜上皮荧光素染色。
- Ponceau 2R-brilliant green double staining technique is a quick,simple and reliable myelin stain method in demonstrating the fasciculi in the central nervous system. 丽春红2R-亮绿法是显示中枢神经纤维束的一种简便、迅速、可靠染色法。
- Conclusions: Ponceau 2R-brilliant green double staining technique is a quick,simple and reliable myelin stain method in demonstrating the fasciculi in the central nervous system. 结论:丽春红2R-亮绿法是显示中枢神经纤维束的一种简便、迅速、可靠染色法。
