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- By using fluorescence staining method, the effect of receptor-mediated endocytosis on intracellular pH (pHi) of mouse macrophages was studied with adherent cell analysis and sorting cytometer 570 (ACAS 570). 用pH值敏感荧光探针Snaflcalcein－AM标记体外培养48小时的小鼠腹腔巨噬细胞，570型粘附式细胞仪观察巨噬细胞在对刀豆素A进行受体介导内吞过程中细胞内pH值的变化。
- Identification of Cause of Hematuria by Application of Immune Fluorescent Staining Method 应用免疫荧光染色方法鉴别血尿的来源
- The apoptosis of suspension cultured cells of Viola diffusa Ging. which had been induced by Ce(NH4)2(NO3)6 were detected by DNA Laddering and DAPI fluorescence stain methods. 用DNA Laddering和DAPI荧光检测法对Ce(NH4)2(NO3)6诱导的蔓茎堇菜(Viola diffusa Ging.;)
- fluorescent staining method 荧光染色法
- MTT method was employed to detect the proliferation of cells,while the adhesive ability was investigated by stereology. In the meantime,the cell integrity and the interface between biomaterial and cells were observed by fluorescent staining and SEM. 采用MTT法检测细胞增殖活性,体视学方法检测细胞粘附能力,荧光双染法检测细胞完整性,扫描电镜观察细胞-材料界面。
- Method lmmunofluorescent histochemical staining method was used. 方法应用免疫荧光组织化学染色方法。
- The innervation of rat thymus was observed by silver stain method. 采用改良的银染色法观察了大鼠胸腺神经纤维的分布。
- Method: An immunohistochemical techniques and SP staining method were used. 方法:免疫组织化学染色。
- Methods Indirect fluorescent staining, FCM analysis and RT PCR were employed to detect the expression and regulation of PTA1 at the levels of PTA1 protein and PTA1mRNA. 用RT PCR方法检测内皮细胞中PTA1mRNA的表达 ;
- Abstract: A practical staining method for counting polyhedra (PIBs) in nucleopolyhedrovirus insecticide was developed. 文摘:本文介绍了一种直接检测病毒杀虫剂产品中多角体数量的染色计数方法。
- Nonspecific and specific phagocytic activities of macrophages with heterogeneous fluorescent staining were observed using starch polysaccharide and immune activators (diphtheria toxoid and BCG) to induce and activate mouse peritoneal macrophages. 利用淀粉多糖和免疫促进剂（白喉类毒素和卡介苗）诱导和活化小鼠腹腔巨噬细胞，观察了四种异质性荧光染色的巨噬细胞非特异性和特异性吞噬活性。
- Immunoreactivities for Fas, FasL and caspase - 3 in-creased considerably in the cervical carcinoma tissue. Dual positive fluorescent staining for ap-optosis and caspase - 3 or Fas was appeared in some cells. 癌组织中，Fas、FasL、caspase－3蛋白阳性细胞明显增多，双重荧光染色显示，部分细胞Fas及cspase－3蛋白与凋亡细胞同时阳性染色。
- At the different end point, the expression of MMP-9 were assayed using immunohistochemistry staining method. 通过免疫组织化学染色方法测定MMP-9的表达。
- Methods For 23 cases (38 eyes) diagnosed of keratoconjunctivitis sicca, conjunctival impression cells were examined by using acridine orange fluorescent staining, the results being evaluated and graded by Tseng Classification. 方法选自我院眼科门诊已确诊的干燥性角结膜炎患者23例(38眼),用丫啶橙荧光染色结膜印迹细胞检查法进行检测,并用结膜印迹细胞检查结果评价标准进行评价,按Tseng分类法进行分级。
- Methods Immunohistochemical technique and SP staining method were used. 免疫组织化学技术,SP染色。
- Methods: IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index (DI) and SPF were calculated. 方法:将IRE-EGFP-esp1质粒用Fu-GENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系(esp1-A549)。 采用DNA荧光染色流式细胞仪分析转染后细胞DNA含量、倍性及细胞周期,计算S期细胞比率(SPF)和DNA指数(DI);
- A practical staining method for counting polyhedra (PIBs) in nucleopolyhedrovirus insecticide was developed. 本文介绍了一种直接检测病毒杀虫剂产品中多角体数量的染色计数方法。
- Aim To evaluate the functional condition of corneal cells and to detect the degree of keratoconjunctivitis sicca using conjunctival impression cell test with acridine orange fluorescent staining. 目的应用丫啶橙荧光染色法进行结膜印迹细胞学检查,通过判断结膜细胞功能状态,对干燥性角结膜炎患者的眼部干燥程度进行定量检测,为临床的治疗提供实验室依据。
- The cell apoptotic rate was measured by flow cytometry (FCM) and Hoechst 33258 DNA staining method. 姜酚化学性质活泼，接触酸、碱、热易发生变性，不易保存。
- Methods Twenty three patients with serious blepharitis were given slit-lamp examination, tear break-up determination, Schemer 1 test and corneal fluorescent staining before and after drug therapy. 方法对23例严重睑缘炎患者进行详细裂隙灯检查、泪膜破裂时间、泪液分泌试验测定及角膜上皮荧光素染色。
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries
