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- Fluorescent quantitation polymerase chain reaction 实时荧光定量PCR
- real time fluorescent quantitation PCR 实时荧光定量PCR
- real-time fluorescent quantitation 实时荧光定量
- real-time fluorescent quantitation RT-PCR 实时荧光定量RT-PCR
- Real-time fluorescent quantitation PCR 实时荧光定量PCR
- Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method. 目的评价荧光定量聚合酶链式反应(FQ-PCR)检测恶性疟原虫的效果。
- Methods HBV markers and HBV-DNA level in the serum were detected by ELISA and fluorescent quantitated PCR. 方法:通过ELISA法及荧光定量PCR法检测血清标本中HBV标志物和HBV-DNA含量水平。
- Real-time fluorescent quantitative PCR(FQ-PCR) is a kind of techniques that can quantitate the nucleic acid based on different fluor escence. 实时荧光定量PCR技术是一种利用不同的荧光检测来给核酸定量的技术。
- real time fluorescent quantitation polymerase chain resction 实时荧光定量聚合酶链反应
- Methods Realtime fluorescent quantitative RT-PCR was used to detect the CK20 mRNA expression in the portal venous blood of 31 patients with colon carcer and 20 healthy volunteers. 方法采用实时荧光定量RT-PCR检测31例病理学确诊的结肠癌患者门静脉血和20例健康对照者外周血CK20mRNA的表达水平。
- Methods: Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect HCV-RNA both in serum and in PBMCs of 65 patients with hepatitis C. 方法:应用荧光定量聚合酶链反应(FQ-PCR)方法,对65例丙肝患者的血清和PBMC中HCV-RNA进行检测。
- Keywords Sexually-transmitted disease;Fluorescent quantitation PCR;Infection; 性传播疾病;荧光定量PCR;感染;
- The retinal function changes were detected by Electroretinogram (ERG). The mRNA expressions of bax, bcl-2, caspase-3 and c-fos were determined by real-time fluorescent quantitative (FQ)-PCR method in rds mice treated with shihusan. 用real-time荧光定量PCR的方法研究石斛散治疗rds小鼠后各时间点凋亡相关基因bax、bcl-2、Caspase-3和c-fos的变化。
- Methods: Brdu-ELISA, Annexin V/PI binding, Real-time fluorescent quantitative RT-PCR, FACS, Electron microscope, Immunocytochemistry and Western blot analysis were applied in present study. 方法 应用的技术包括BrdU-ELISA、Annexin V/PI染色、透射电镜、实时荧光定量RT-PCR、流式细胞术、免疫细胞化学和Western blot等。
- Real-time fluorescent quantitative PCR basing ordinary PCR is a new technique which possess characteristic of sensitiveness,quickness and speciality,can accurately fix the quality. 实时荧光定量PCR是在普通PCR基础上发展起来的新技术,在保持了PCR技术灵敏、快速、特异特点的基础上增加了定量的特点。
- Objective:To establish fluorescence quantitative nested RT-PCR method for detecting Borna disease virus (BDV). 目的 :建立博尔纳病病毒 (BDV)荧光定量套式RT -PCR检测方法 ,为快速、准确地定量检测BDV奠定基础。
- Fluorescence quantitative PCR combined with RT-PCR technique can detect the DTC in blood of the patients with HCC. RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散的肿瘤细胞,并且以肺动脉血中阳性率最高。
- Detection deleted Duchenne muscular dystrophy carriers by fluorescence quantitative polymerase chain reaction. 应用荧光定量PCR检测缺失型DMD携带者的研究
- Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis. 目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。
- Objective:To construct recombinant plasmid pMD18-nm23-H1/p as the standards for nm23-H1 gene detection by real-time fluorescence quantitative PCR. 目的:制备用于nm23-H1基因mRNA实时荧光定量PCR检测的质粒标准品。