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- feeder layer technique 饲养层技术
- The feasibility of Confined Etchant Layer Technique(CELT) has been studied. 本文对用于三维超出图形复制加工的约束刻蚀剂层技术进行了可行性研究。
- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering. 在复苏后早期阶段ES细胞培养中 ,不宜单独应用BRL条件培养基 ,须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层
- MEF, bEF, hEF feeder layer cells all can promote the efficiency of the isolation and clone human ES-like cells derived from PGCs. MEF、bEF、hEF三种饲养层细胞对人PGCs体外培养均有促进作用。
- CONCLUSION: Mouse blastula can develop into embryonic stem cells on the fibroblast cell feeder layer, and can be passaged. 结论：小鼠囊胚在胚胎成纤维细胞饲养层上可发育成胚胎干细胞，并能进行传代。
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词：胚胎成纤维细胞；
- Electrochemical etching of single crystal GaAs (111) was performed with pyramid-like microstructure arrays by confined etchant layer technique (CELT). 以“金字塔”状的锥体阵列作为电化学模板,利用约束刻蚀剂层技术对半导体GaAs进行加工刻蚀。
- CONCLUSION: Endometrial fibroblast is considered to be feeder layer of PGC.After passage, PGC remains undifferentiation and pluripotent of embryonic stem cells. 结论：人子宫内膜成纤维细胞可以作为人原始生殖细胞的饲养层细胞，传代后的原始生殖细胞仍保持胚胎干细胞的未分化状态和发育全能性。
- The chemical microetching on copper surface with three-dimensional(3D) gear-like molds was realized by confined etchant layer technique(CELT). 运用约束刻蚀剂层技术 (CELT)在金属铜 (Cu)表面实现了三维微图形加工 ,取得成功的关键因素在于寻找到能对Cu进行有效CELT加工的化学刻蚀和捕捉体系。
- MAIN OUTCOME MEASURES: Preparation of fibroblast feeder layer;growth of embryonic stem cells;results of alkaline phosphatase staining;cell differentiation tendency. 主要观察指标：成纤维细胞饲养层的制备情况，胚胎干细胞的生长状态、碱性磷酸酶染色结果及分化趋势。
- This article presents a discussion on the feasibility of using dye- gelatine type anti- halation layer technique in aerial photographic film. 本文主要论述了凝胶型防光晕层在军事航空摄影胶片中应用的可行性，通过实验解决了一系列关键技术，通过生产实践进行验证。
- Under appropriate conditions, the adherent cells from HUCB could form the feeder layer to support long-term hematopoiesis and provide the supporting stromal cells for the ex vivo expansion of HSPCs. 在适当的条件下;可以形成饲养层细胞;为造血干/祖细胞体外扩增提供支持基质细胞.
- Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. 方法 将外源性neo基因转染人胚胎成纤维细胞 ,作为饲养层细胞用于ES细胞转基因后筛选及增殖过程的培养。
- When cultured on degenerated feeder layers or in suspension, EG cells formed embryoid bodies (EBs) in vitro. 当EG细胞脱离饲养层悬浮培养，或在衰老的饲养层上延迟培养时，发现EG细胞或单个存在，或聚集成团，形成类似于早期胚胎的囊状胚体结构.
- We use the orbit trap tracing, color palette and layering technique to produce 3D fractal image which has a fascinated appearance. 我们采用轨迹井跟踪、调色板和图层技术来生成这类具有艺术感染力的3D分形图像,并且生成算法简单、快速。
- METHODS: PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture. 方法：从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
- Confined etchant layer technique (CELT) 约束刻蚀剂层技术
- The little boy is a noisy feeder. 这个小男孩吃东西时发出响声。
- Confined etchant layer technique 约束刻蚀剂层技术
