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- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering. 在复苏后早期阶段ES细胞培养中 ,不宜单独应用BRL条件培养基 ,须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层
- MEF, bEF, hEF feeder layer cells all can promote the efficiency of the isolation and clone human ES-like cells derived from PGCs. MEF、bEF、hEF三种饲养层细胞对人PGCs体外培养均有促进作用。
- CONCLUSION: Mouse blastula can develop into embryonic stem cells on the fibroblast cell feeder layer, and can be passaged. 结论：小鼠囊胚在胚胎成纤维细胞饲养层上可发育成胚胎干细胞，并能进行传代。
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词：胚胎成纤维细胞；
- CONCLUSION: Endometrial fibroblast is considered to be feeder layer of PGC.After passage, PGC remains undifferentiation and pluripotent of embryonic stem cells. 结论：人子宫内膜成纤维细胞可以作为人原始生殖细胞的饲养层细胞，传代后的原始生殖细胞仍保持胚胎干细胞的未分化状态和发育全能性。
- MAIN OUTCOME MEASURES: Preparation of fibroblast feeder layer;growth of embryonic stem cells;results of alkaline phosphatase staining;cell differentiation tendency. 主要观察指标：成纤维细胞饲养层的制备情况，胚胎干细胞的生长状态、碱性磷酸酶染色结果及分化趋势。
- Under appropriate conditions, the adherent cells from HUCB could form the feeder layer to support long-term hematopoiesis and provide the supporting stromal cells for the ex vivo expansion of HSPCs. 在适当的条件下;可以形成饲养层细胞;为造血干/祖细胞体外扩增提供支持基质细胞.
- Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. 方法 将外源性neo基因转染人胚胎成纤维细胞 ,作为饲养层细胞用于ES细胞转基因后筛选及增殖过程的培养。
- When cultured on degenerated feeder layers or in suspension, EG cells formed embryoid bodies (EBs) in vitro. 当EG细胞脱离饲养层悬浮培养，或在衰老的饲养层上延迟培养时，发现EG细胞或单个存在，或聚集成团，形成类似于早期胚胎的囊状胚体结构.
- METHODS: PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture. 方法：从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
- stroma feeder layer 基质滋养层
- The little boy is a noisy feeder. 这个小男孩吃东西时发出响声。
- Under the same culture condition, nest-like colonies were observedonly from l9dpc and 2odpc fetuses in SBR and DEB rabbits when rabbit PGCs were seededonto homologous feeder layers. 19-20dpc塞北兔和大耳白兔胎儿与同期的新西兰大白兔胎儿相比，由PGCs建成的EG细胞系在相同培养条件下易于分化。
- Therefore, the parthenogenetic morulas without zona pellucida are more capable of attaching onto the feeder layers of PMEFs than the parthenogenetic blastocysts with intact zona pellucida. 说明去透明带孤雌桑葚胚比留透明带孤雌囊胚更利于在饲养层上贴壁生长，为建立孤雌生殖的胚胎干细胞系提供了基础。
- This layer of rock contains a lot of flint. 这一岩层中有大量燧石。
- Preparation of the feeder layer of mouse embryonic stem cells 小鼠胚胎干细胞成纤维细胞液养层的制备
- The inner layer of the wall of a spore. 孢子内壁孢子壁的内层
- There is a fine layer of dust on the desk. 桌子上有一层薄薄的尘土。
- Reserve cells possibly were originated from stroma. 储备细胞可能来源于间质。
